Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody - Loading Control

Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK)
Permeabilization
Yes - 0.5% Triton X-100
Specification
MDCK
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Invitrogen Alexa Fluor 633
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647

Additional data

Additional Notes
Using this antibody you can see the signals but you can not see the fibers of MT. Maybe we didn't find the best condition or maybe this antibody is not suitable if you want to see the fibers of MT. A pseudo-colour (cyan) is used to represent the far-red emission (633).
Abcam response
Thank you very much for your honest feedback about the use of ab89984 in dog. This species has not been tested before and as the customer suggest, the antibody may not suitable for dog under this experimental conditions. We welcome any further feedback and Abreviews from other customers who have used this antibody in dog which will help us assess how well the antibody works in this species.

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Verified customer

Submitted Feb 29 2016

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