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  1. Link

    alpha-tubulin-antibody-microtubule-marker-ab15246.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)

  • Datasheet
  • SDS
Reviews (18) Submit a question References (222)

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Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
  • Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)

Key features and details

  • Rabbit polyclonal to alpha Tubulin - Microtubule Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-alpha Tubulin antibody - Microtubule Marker
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit polyclonal to alpha Tubulin - Microtubule Marker
  • Host species

    Rabbit
  • Specificity

    Homology to both Tubulin alpha-3E chain and Tubulin alpha-4A chain

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Drosophila melanogaster, Zebrafish, Gerbil, Newt
  • Immunogen

    Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q6PEY2

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Alpha Tubulin
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • SK-N-SH whole cell lysate (ab3956)
  • Related Products

    • alpha Tubulin peptide (ab195584)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (14)
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P (1)
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Entrez Gene: 316531 Rat
    • Entrez Gene: 573122 Zebrafish
    • Entrez Gene: 573216 Zebrafish
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • SwissProt: Q5XIF6 Rat
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • Unigene: 92961 Rat
    see all
  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
    Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)

    Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa

    Lanes: 1-3: 4oC (1 freeze/thaw);  4-6: 4oC (2 freeze/thaws); 7-9 :   4oC (4 freeze/thaws) 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)

    ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections). 

  • Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
    Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
    All lanes : Anti-alpha Tubulin antibody - Microtubule Marker (ab15246) at 1 µg/ml

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 50 kDa
    Observed band size: 55 kDa why is the actual band size different from the predicted?

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (222)

Publishing research using ab15246? Please let us know so that we can cite the reference in this datasheet.

ab15246 has been referenced in 222 publications.

  • Chen XK  et al. 1-phenyl 2-thiourea (PTU) activates autophagy in zebrafish embryos. Autophagy 17:1222-1231 (2021). PubMed: 32286915
  • Lafouresse F  et al. Stochastic asymmetric repartition of lytic machinery in dividing CD8+ T cells generates heterogeneous killing behavior. Elife 10:N/A (2021). PubMed: 33427199
  • Rashnonejad A  et al. Designed U7 snRNAs inhibit DUX4 expression and improve FSHD-associated outcomes in DUX4 overexpressing cells and FSHD patient myotubes. Mol Ther Nucleic Acids 23:476-486 (2021). PubMed: 33510937
  • Strausfeld N & Sayre ME Shore crabs reveal novel evolutionary attributes of the mushroom body. Elife 10:N/A (2021). PubMed: 33559601
  • Tavares-Valente D  et al. Disruption of pH Dynamics Suppresses Proliferation and Potentiates Doxorubicin Cytotoxicity in Breast Cancer Cells. Pharmaceutics 13:N/A (2021). PubMed: 33572458
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 18 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Swine Testicle (ST) cells infected with pseudorabi)
Permeabilization
Yes - 0.1% Triton-X
Specification
Swine Testicle (ST) cells infected with pseudorabi
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 30 2020

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
THP-1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 22 2019

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10000 cells
Specification
Glioblastoma
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Gilberto Betancor Quintana

Verified customer

Submitted Jun 20 2019

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 08 2018

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Glioblastoma (GL261))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10000 cells
Treatment
HDAC inhibitors for 24hrs
Specification
Mouse Glioblastoma (GL261)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 12 2014

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Non-reduced Denaturing (12.5)
Loading amount
20 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 28 2013

Immunocytochemistry abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Permeabilization
Yes - triton X-100
Specification
HeLa
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 15°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. AeLee Jeong

Verified customer

Submitted Apr 05 2013

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (High-fat diet-fed SD rat muscle)
Gel Running Conditions
Reduced Denaturing (9% SDS-PAGE)
Loading amount
30 µg
Specification
High-fat diet-fed SD rat muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 19 2013

Immunoprecipitation abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293 T)
Total protein in input
100 µg
Immuno-precipitation step
Protein A/G
Specification
HEK 293 T
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Chris Tracy

Verified customer

Submitted Sep 18 2012

Western blot abreview for Anti-alpha Tubulin antibody - Microtubule Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions
Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Loading amount
100000 cells
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 23 2012

1-10 of 18 Abreviews or Q&A

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