Recombinant

Recombinant Anti-alpha Tubulin antibody [RM113] - C-terminal (Biotin) (ab222758)

Overview

  • Product name

    Anti-alpha Tubulin antibody [RM113] - C-terminal (Biotin)
    See all alpha Tubulin primary antibodies
  • Description

    Rabbit monoclonal [RM113] to alpha Tubulin - C-terminal (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Specificity

    This antibody reacts with alpha tubulin, including both tubulin alpha-1A chain and tubulin alpha-1B chain.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity


    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide corresponding to Human alpha Tubulin (C terminal).

  • Positive control

    • ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) cells. WB: A431 (human epidermoid carcinoma cell line) cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab222758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

Images

  • Anti-alpha Tubulin antibody [RM113] - C-terminal (Biotin) (ab222758) at 1/1000 dilution + A431 cell lysate
  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for alpha Tubulin (red) using ab222758 at a 1/200 dilution in ICC/IF. Nuclei counterstained with DAPI (blue).

References

ab222758 has not yet been referenced specifically in any publications.

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