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    alpha-tubulin-antibody-tu-01-ab7750.pdf

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Tags & Cell Markers Subcellular Markers Cytoskeleton Microtubules
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Anti-alpha Tubulin antibody [TU-01] (ab7750)

  • Datasheet
  • SDS
Reviews (8) Submit a question References (23)

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Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Flow Cytometry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
  • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

Key features and details

  • Mouse monoclonal [TU-01] to alpha Tubulin
  • Suitable for: ICC, Flow Cyt, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-alpha Tubulin antibody [TU-01]
    See all alpha Tubulin primary antibodies
  • Description

    Mouse monoclonal [TU-01] to alpha Tubulin
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Mouse
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length native protein (purified) corresponding to Pig alpha Tubulin aa 37-154.

  • Epitope

    aa 65-97 on N-terminal structural domain
  • Positive control

    • ICC: HeLa and NIH/3T3 cells. IHC-P: Human skin tissue. Flow Cyt: HEK293 cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    TU-01
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Alpha Tubulin

Associated products

  • Alternative Versions

    • Biotin Anti-alpha Tubulin antibody [TU-01] (ab74696)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Related Products

    • alpha Tubulin peptide (ab195584)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Mouse
ICC/IF
Mouse
Human
IHC-P
Human
WB
Human
All applications
Turkey
Pig
Saccharomyces cerevisiae
Paramecium tetraurelia
Nicotiana benthamiana
Application Abreviews Notes
ICC
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
ICC
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • Alternative names

      • Alpha-tubulin 1 antibody
      • ALS22 antibody
      • B ALPHA 1 antibody
      • bA408E5.3 antibody
      • H2 ALPHA antibody
      • Hum a tub1 antibody
      • Hum a tub2 antibody
      • LIS3 antibody
      • MGC171407 antibody
      • MGC55332 antibody
      • TBA4A_HUMAN antibody
      • Testis-specific alpha-tubulin antibody
      • TUBA1 antibody
      • TUBA1A antibody
      • tuba1l antibody
      • Tuba4a antibody
      • Tubulin alpha 1 chain antibody
      • Tubulin alpha antibody
      • Tubulin alpha-1 chain antibody
      • tubulin alpha-1B chain antibody
      • Tubulin alpha-4A chain antibody
      • Tubulin H2-alpha antibody
      • Tubulin, alpha 1 (testis specific) antibody
      • tubulin, alpha 1, like antibody
      • Tubulin, alpha 4a antibody
      • Tubulin, alpha, testis-specific antibody
      • Tubulin, alpha-1 antibody
      see all

    Images

    • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      ICC/IF image of ab7750 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7750, 5µg/ml) overnight at +4°C. The secondary antibody (green) was anti-mouse DyLight® 488 (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Ab7750 staining human normal skin. Staining is localised to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with a retrieval buffer EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with an amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    • Flow Cytometry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Flow Cytometry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Overlay histogram showing HEK293 cells stained with ab7750 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7750, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was anti-mouse DyLight® 488 (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

    • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry/ Immunofluorescence analysis of NIH 3T3 (mouse embryonal fibroblast) cells labelling alpha tubulin (green) with ab7750. Vimentin was stained red as counterstain. DAPI was used to stain the nuclei blue. 
    • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Immunocytochemistry analysis of HeLa (human cervix carcinoma) cells labelling alpha tubulin (red) with ab7750. DAPI was used to stain the nuclei blue. 

    • Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)
      Immunocytochemistry - Anti-alpha Tubulin antibody [TU-01] (ab7750)

      Immunocytochemistry analysis of NIH 3T3 (mouse embryonal fibroblast) cells labelling alpha tubulin (green) with ab7750. DAPI was used to stain the nuclei blue. 

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (23)

    Publishing research using ab7750? Please let us know so that we can cite the reference in this datasheet.

    ab7750 has been referenced in 23 publications.

    • Katsumata Y  et al. Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene. J Neuropathol Exp Neurol 79:3-21 (2020). PubMed: 31748784
    • Sudevan S  et al. Mitochondrial dysfunction causes Ca2+ overload and ECM degradation-mediated muscle damage in C. elegans. FASEB J 33:9540-9550 (2019). PubMed: 31162948
    • Wei J  et al. Anti-proliferative effect of isorhamnetin on HeLa cells through inducing G2/M cell cycle arrest. Exp Ther Med 15:3917-3923 (2018). PubMed: 29563987
    • Refolo V  et al. Progressive striatonigral degeneration in a transgenic mouse model of multiple system atrophy: translational implications for interventional therapies. Acta Neuropathol Commun 6:2 (2018). PubMed: 29298733
    • Elkis Y  et al. A novel Fer/FerT targeting compound selectively evokes metabolic stress and necrotic death in malignant cells. Nat Commun 8:940 (2017). PubMed: 29038547
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-alpha Tubulin antibody [TU-01]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (oral epithelial)
    Specification
    oral epithelial
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Tina Yuan

    Verified customer

    Submitted Sep 26 2018

    Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Plants Tissue lysate - whole (Nicotiana benthamiana)
    Gel Running Conditions
    Non-reduced Denaturing (4-20% precut gels)
    Loading amount
    10 µg
    Treatment
    lysis buffer 12h
    Specification
    Nicotiana benthamiana
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 20°C
    Read More

    Huan Zhang

    Verified customer

    Submitted Nov 13 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin [TU-01] antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Sample
    Plants Cell (meiocyte)
    Specification
    meiocyte
    Permeabilization
    Yes - Triton X-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 23 2014

    Western blot abreview for Anti-alpha Tubulin [TU-01] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - other (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 17 2014

    Immunoprecipitation abreview for Anti-alpha Tubulin antibody [TU-01]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK 293T)
    Total protein in input
    350 µg
    Specification
    HEK 293T
    Immuno-precipitation step
    Protein A/G
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 26 2012

    Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hek 293)
    Loading amount
    10 µg
    Specification
    Hek 293
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
    Read More

    Dr. Chris Tracy

    Verified customer

    Submitted Jan 12 2012

    Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (forebrain)
    Loading amount
    10 µg
    Specification
    forebrain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 19 2007

    Western blot abreview for Anti-alpha Tubulin antibody [TU-01]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Tissue lysate - whole (Heart)
    Loading amount
    80 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Dr. sudhish mishra

    Verified customer

    Submitted Apr 27 2007

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