Overview

  • Product name

    Anti-alpha Tubulin antibody [TU-01] (Biotin)
    See all alpha Tubulin primary antibodies
  • Description

    Mouse monoclonal [TU-01] to alpha Tubulin (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    ab74696 recognizes the defined epitope (aa 65-97) on the N-terminal structural domain of alpha-tubulin. This epitope is conserved within all species.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.

  • General notes

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

Properties

Applications

Our Abpromise guarantee covers the use of ab74696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining. PubMed: 25665878
WB 1/1000. Predicted molecular weight: 54 kDa.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Sequence similarities

    Belongs to the tubulin family.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

References

ab74696 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and your interest in our products. Anti-alpha tubulin (clone TU-01) can be used as loading control together with antibody HLA Class I (ab23755) . Currently, we have several forms of this antibody ie. unconjugated as well as conjugated: - ab7750 - Anti-alpha Tubulin antibody [TU-01] - ab18247 - Anti-alpha Tubulin antibody [TU-01] (FITC) - ab46708 - Anti-alpha Tubulin antibody [TU-01] (Dyomics® 547) - ab74696 - Anti-alpha Tubulin antibody [TU-01] (Biotin) - ab78108 - Anti-alpha Tubulin antibody [TU-01] I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question
Answer

I think my first guess when we spoke was 3-6 biotins per IgG, but the lab says 4-8. I hope this helps. If you are trying to compare the relative signal you can expect from different anti-tubulin antibodies, this followed by an avidin or streptavidn conjugate should be more sensitive than an anti-primary IgG secondary antibody conjugated to the same fluorochrome or enzyme.

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