Product nameAnti-Alpha1-adaptin antibody [EPR7572]
See all Alpha1-adaptin primary antibodies
DescriptionRabbit monoclonal [EPR7572] to Alpha1-adaptin
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Alpha1-adaptin aa 50-150. The exact sequence is proprietary.
Database link: O95782
- HeLa, 293T, HepG2 and A431 cell lysates; Human prostate and liver tissues; Hela cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab151720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 108 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
All lanes : Anti-Alpha1-adaptin antibody [EPR7572] (ab151720) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 108 kDa
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling alpha Adaptin with ab151720 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling alpha Adaptin with ab151720 at 1/50 dilution.
Flow cytometric analysis of permeabilized HeLa cells labeling alpha Adaptin with ab151720 at 1/100 diluton (red), compared to a nonspecific control antibody (green).
ab151720 has not yet been referenced specifically in any publications.