Overview

  • Product name

    Anti-Alpha1-adaptin antibody [EPR7572]
    See all Alpha1-adaptin primary antibodies
  • Description

    Rabbit monoclonal [EPR7572] to Alpha1-adaptin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Alpha1-adaptin aa 50-150. The exact sequence is proprietary.
    Database link: O95782

  • Positive control

    • HeLa, 293T, HepG2 and A431 cell lysates; Human prostate and liver tissues; Hela cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7572
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Images

    • All lanes : Anti-Alpha1-adaptin antibody [EPR7572] (ab151720) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 108 kDa

    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling alpha Adaptin with ab151720 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling alpha Adaptin with ab151720 at 1/50 dilution.
    • Flow cytometric analysis of permeabilized HeLa cells labeling alpha Adaptin with ab151720 at 1/100 diluton (red), compared to a nonspecific control antibody (green).

    References

    ab151720 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151720.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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