Overview

  • Product name

  • Description

    Mouse polyclonal to ALPK1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human ALPK1 protein, amino acids 1-1085 (AAH60780.1)

  • Positive control

    • HepG2 cell lysate, ALPK1 transfected 293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 119 kDa (predicted molecular weight: 139 kDa).

Target

  • Function

    Kinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
  • Tissue specificity

    Highly expressed in liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
    Contains 1 alpha-type protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 8430410J10Rik antibody
    • Alpha protein kinase 1 antibody
    • Alpha-protein kinase 1 antibody
    • ALPK1 antibody
    • ALPK1_HUMAN antibody
    • Chromosome 4 kinase antibody
    • LAK antibody
    • Lymphocyte alpha kinase antibody
    • Lymphocyte alpha protein kinase antibody
    • Lymphocyte alpha-protein kinase antibody
    • RGD1304613 antibody
    see all

Images

  • Anti-ALPK1 antibody (ab89140) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size: 139 kDa
    Observed band size: 119 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.

  • All lanes : Anti-ALPK1 antibody (ab89140) at 1 µg/ml

    Lane 1 : ALPK1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 139 kDa
    Observed band size: 170 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Yamada Y  et al. Identification of chromosome 3q28 and ALPK1 as susceptibility loci for chronic kidney disease in Japanese individuals by a genome-wide association study. J Med Genet 50:410-8 (2013). Read more (PubMed: 23539754) »
See 1 Publication for this product

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