Product nameAnti-ALPPL2 antibody [EPR20905-21] - BSA and Azide free
See all ALPPL2 primary antibodies
DescriptionRabbit monoclonal [EPR20905-21] to ALPPL2 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human ALPPL2 aa 50-150. The exact sequence is proprietary.
Database link: P10696
- WB: PANC-1, Capan-1 and nES whole cell lysates.
ab269957 is the carrier-free version of ab235581. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab269957 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab269957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).|
Tissue specificityTrace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.
Sequence similaritiesBelongs to the alkaline phosphatase family.
Cellular localizationCell membrane.
- Information by UniProt
- Akp5 antibody
- Alkaline phosphatase antibody
- Alkaline phosphatase Nagao isozyme antibody
All lanes : Anti-ALPPL2 antibody [EPR20905-21] (ab235581) at 1/1000 dilution
Lane 1 : nES (human native embryonic stem cell), whole cell lysate
Lane 2 : pES (human primed embryonic stem cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were kindly provided by Dr Shaorong Gao.
140KD band is dimer (PMID: 23467613).
Exposure time: 5.5 seconds.
These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab235581).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab269957 has not yet been referenced specifically in any publications.