Anti-ALR antibody (ab36376)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-ALR antibody
See all ALR primary antibodies -
DescriptionRabbit polyclonal to ALR
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human ALR.
Read Abcam's proprietary immunogen policy (Peptide available as ab36485.) -
Positive control
- Purchase matching WB positive control:Recombinant Human ALR protein
- Recombinant Human ALR protein (ab114811) can be used as a positive control in WB. This antibody gave a positive signal in mouse liver and mouse testis tissue lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab36376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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FunctionIsoform 1: FAD-dependent sulfhydryl oxidase. Within the mitochondrial intermembrane space, participates in a chain of disulfide exchange reactions with MIA40, that generate disulfide bonds in a number of resident proteins with twin Cx3C and Cx9C motifs.
Isoform 2: May act as an autocrine hepatotrophic growth factor promoting liver regeneration. -
Tissue specificityUbiquitously expressed. Highest expression in the testis and liver and low expression in the muscle.
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Involvement in diseaseDefects in GFER are a cause of mitochondrial progressive myopathy with congenital cataract hearing loss and developmental delay (MPMCHD) [MIM:613076]; also called combined mitochondrial complex deficiency.
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Sequence similaritiesContains 1 ERV/ALR sulfhydryl oxidase domain.
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Cellular localizationMitochondrion intermembrane space and Cytoplasm. Secreted.
- Information by UniProt
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Database links
- Entrez Gene: 2671 Human
- Entrez Gene: 11692 Mouse
- Entrez Gene: 27100 Rat
- Omim: 600924 Human
- SwissProt: P55789 Human
- SwissProt: P56213 Mouse
- SwissProt: Q63042 Rat
- Unigene: 726325 Human
see all -
Alternative names
- ALR_HUMAN antibody
- Augmenter of liver regeneration antibody
- ERV1 antibody
see all
Images
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Western blot - Anti-ALR antibody (ab36376)All lanes : Anti-ALR antibody (ab36376) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-ALR antibody (ab36376)ICC/IF image of ab36376 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36376, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
Datasheets and documents
References
ab36376 has not yet been referenced specifically in any publications.
