Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab36376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Isoform 1: FAD-dependent sulfhydryl oxidase. Within the mitochondrial intermembrane space, participates in a chain of disulfide exchange reactions with MIA40, that generate disulfide bonds in a number of resident proteins with twin Cx3C and Cx9C motifs.
    Isoform 2: May act as an autocrine hepatotrophic growth factor promoting liver regeneration.
  • Tissue specificity
    Ubiquitously expressed. Highest expression in the testis and liver and low expression in the muscle.
  • Involvement in disease
    Defects in GFER are a cause of mitochondrial progressive myopathy with congenital cataract hearing loss and developmental delay (MPMCHD) [MIM:613076]; also called combined mitochondrial complex deficiency.
  • Sequence similarities
    Contains 1 ERV/ALR sulfhydryl oxidase domain.
  • Cellular localization
    Mitochondrion intermembrane space and Cytoplasm. Secreted.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ALR_HUMAN antibody
    • Augmenter of liver regeneration antibody
    • ERV1 antibody
    • ERV1 homolog antibody
    • Erv1 like growth factor antibody
    • FAD-linked sulfhydryl oxidase ALR antibody
    • GFER antibody
    • Growth factor augmenter of liver regeneration antibody
    • Growth factor erv1 like antibody
    • Hepatic regenerative stimulation substance antibody
    • Hepatopoietin antibody
    • Hepatopoietin protein antibody
    • hERV1 antibody
    • HPO antibody
    • HPO1 antibody
    • HPO2 antibody
    • HSS antibody
    see all

Images

  • Western blot - Anti-ALR antibody (ab36376)
    Western blot - Anti-ALR antibody (ab36376)
    All lanes : Anti-ALR antibody (ab36376) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunocytochemistry/ Immunofluorescence - Anti-ALR antibody (ab36376)
    Immunocytochemistry/ Immunofluorescence - Anti-ALR antibody (ab36376)
    ICC/IF image of ab36376 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36376, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.

References

ab36376 has not yet been referenced specifically in any publications.

Publishing research using ab36376? Please let us know so that we can cite the reference in this datasheet.

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