Key features and details
- Rabbit polyclonal to ALR/HPO
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ALR/HPO antibody
See all ALR/HPO primary antibodies
DescriptionRabbit polyclonal to ALR/HPO
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis
- Purchase matching WB positive control:Recombinant Human ALR/HPO protein
- Recombinant Human ALR/HPO protein (ab114811) can be used as a positive control in WB. This antibody gave a positive signal in mouse liver and mouse testis tissue lysates.
This product was previously labelled as ALR
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionIsoform 1: FAD-dependent sulfhydryl oxidase. Within the mitochondrial intermembrane space, participates in a chain of disulfide exchange reactions with MIA40, that generate disulfide bonds in a number of resident proteins with twin Cx3C and Cx9C motifs.
Isoform 2: May act as an autocrine hepatotrophic growth factor promoting liver regeneration.
Tissue specificityUbiquitously expressed. Highest expression in the testis and liver and low expression in the muscle.
Involvement in diseaseDefects in GFER are a cause of mitochondrial progressive myopathy with congenital cataract hearing loss and developmental delay (MPMCHD) [MIM:613076]; also called combined mitochondrial complex deficiency.
Sequence similaritiesContains 1 ERV/ALR sulfhydryl oxidase domain.
Cellular localizationMitochondrion intermembrane space and Cytoplasm. Secreted.
- Information by UniProt
- ALR_HUMAN antibody
- Augmenter of liver regeneration antibody
- ERV1 antibody
All lanes : Anti-ALR/HPO antibody (ab36376) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab36376 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36376, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
ab36376 has not yet been referenced specifically in any publications.