Product nameAnti-Als2 antibody [EPR11185]
See all Als2 primary antibodies
DescriptionRabbit monoclonal [EPR11185] to Als2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human Als2 aa 250-350 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96Q42
- SH-SY5Y, 293T and HeLa cell lysates; Human brain and pancreas tissues; SH-SY5Y cells; 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 184 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay act as a GTPase regulator. Controls survival and growth of spinal motoneurons.
Involvement in diseaseDefects in ALS2 are the cause of amyotrophic lateral sclerosis type 2 (ALS2) [MIM:205100]. ALS2 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
Defects in ALS2 are the cause of juvenile primary lateral sclerosis (JPLS) [MIM:606353]. JPLS is a neurodegenerative disorder which is closely related to but clinically distinct from amyotrophic lateral sclerosis. It is a progressive paralytic disorder which results from dysfunction of the upper motor neurons of the motor cortex while the lower neurons are unaffected.
Defects in ALS2 are the cause of infantile-onset ascending spastic paralysis (IAHSP) [MIM:607225]. IAHSP is characterized by progressive spasticity and weakness of limbs.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 8 MORN repeats.
Contains 1 PH domain.
Contains 5 RCC1 repeats.
Contains 1 VPS9 domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- ALS 2 antibody
- ALS2 antibody
- ALS2_HUMAN antibody
Flow cytometric analysis of permeabilized 293T cells labeling Als2 with ab170896 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunofluorescent analysis of SH-SY5Y cells labeling Als2 with ab170896 at 1/50 dilution (red). DAPI nuclear staining (blue).
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Als2 with ab170896 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Als2 with ab170896 at 1/50 dilution.
All lanes : Anti-Als2 antibody [EPR11185] (ab170896) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 184 kDa
ab170896 has not yet been referenced specifically in any publications.