Overview

  • Product name
    Anti-Als2 antibody [EPR11185]
    See all Als2 primary antibodies
  • Description
    Rabbit monoclonal [EPR11185] to Als2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Als2 aa 250-350 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96Q42

  • Positive control
    • SH-SY5Y, 293T and HeLa cell lysates; Human brain and pancreas tissues; SH-SY5Y cells; 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 184 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May act as a GTPase regulator. Controls survival and growth of spinal motoneurons.
    • Involvement in disease
      Defects in ALS2 are the cause of amyotrophic lateral sclerosis type 2 (ALS2) [MIM:205100]. ALS2 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
      Defects in ALS2 are the cause of juvenile primary lateral sclerosis (JPLS) [MIM:606353]. JPLS is a neurodegenerative disorder which is closely related to but clinically distinct from amyotrophic lateral sclerosis. It is a progressive paralytic disorder which results from dysfunction of the upper motor neurons of the motor cortex while the lower neurons are unaffected.
      Defects in ALS2 are the cause of infantile-onset ascending spastic paralysis (IAHSP) [MIM:607225]. IAHSP is characterized by progressive spasticity and weakness of limbs.
    • Sequence similarities
      Contains 1 DH (DBL-homology) domain.
      Contains 8 MORN repeats.
      Contains 1 PH domain.
      Contains 5 RCC1 repeats.
      Contains 1 VPS9 domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALS 2 antibody
      • ALS2 antibody
      • ALS2_HUMAN antibody
      • ALS2CR6 antibody
      • Alsin antibody
      • ALSJ antibody
      • Amyotrophic lateral sclerosis 2 (juvenile) antibody
      • Amyotrophic lateral sclerosis 2 (juvenile) chromosome region candidate 6 antibody
      • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 6 protein antibody
      • Amyotrophic lateral sclerosis 2 protein antibody
      • Amyotrophic lateral sclerosis protein 2 antibody
      • FLJ31851 antibody
      • IAHSP antibody
      • KIAA1563 antibody
      • MGC87187 antibody
      • PLSJ antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized 293T cells labeling Als2 with ab170896 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    • Immunofluorescent analysis of SH-SY5Y cells labeling Als2 with ab170896 at 1/50 dilution (red). DAPI nuclear staining (blue).

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Als2 with ab170896 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Als2 with ab170896 at 1/50 dilution.

    • All lanes : Anti-Als2 antibody [EPR11185] (ab170896) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 184 kDa

    References

    ab170896 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblasts)
    Permeabilization
    Yes - Triton X-100 in blocking buffer
    Specification
    fibroblasts
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Nov 20 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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