Anti-ALS2CR12 antibody (ab237687)

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Overview

  • Product name

    Anti-ALS2CR12 antibody
    See all ALS2CR12 primary antibodies

  • Description

    Rabbit polyclonal to ALS2CR12

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human ALS2CR12 aa 26-76.
    Sequence:

    PQLPRKNSTGSSKLTPLVPAPKNHNYLQPTKPVVSPKMKIHSARQEETNK S


    Database link: Q96Q35

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, A549 and PC-3 whole cell lysate. ICC/IF: A549 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purity greater than 95%.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab237687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000.
ICC/IF 1/50 - 1/200.

Target

  • Relevance

    The exact function of ALS2CR12 remains unknown.

  • Database links

    • Alternative names

      • ALS2CR12 antibody
      • Amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 12 antibody
      • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody

    Images

    • Western blot - Anti-ALS2CR12 antibody (ab237687)
      Western blot - Anti-ALS2CR12 antibody (ab237687)
      All lanes : Anti-ALS2CR12 antibody (ab237687) at 1/500 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

      Secondary
      All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
    • Immunocytochemistry/ Immunofluorescence - Anti-ALS2CR12 antibody (ab237687)
      Immunocytochemistry/ Immunofluorescence - Anti-ALS2CR12 antibody (ab237687)

      A549 (Human lung carcinoma cell line) cells stained for ALS2CR12 (Green) using ab237687 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

      The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

    References

    ab237687 has not yet been referenced specifically in any publications.

    Publishing research using ab237687? Please let us know so that we can cite the reference in this datasheet.

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