Product nameAnti-ALS2CR8 antibody [EPR13712]
See all ALS2CR8 primary antibodies
DescriptionRabbit monoclonal [EPR13712] to ALS2CR8
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant fragment within Human ALS2CR8 aa 100-300. The exact sequence is proprietary.
Database link: Q8N187
- SH-SY5Y and 293T cell lysate, SH-SY5Y cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionActs as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3' sequence.
DomainThe N-terminus is necessary for DNA-binding. The C-terminus is necessary for transcriptional activation (PubMed:11832226).
- Information by UniProt
- Amyotrophic lateral sclerosis 2 (juvenile) chromosome region candidate 8 antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein antibody
- Calcium response factor antibody
Flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling ALS2CR8 with ab180142 at 1/90. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG
All lanes : Anti-ALS2CR8 antibody [EPR13712] (ab180142) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 81 kDa
Observed band size: 81 kDa
Blocking buffer and concentration: 5% NFDM/TBST
ab180142 has not yet been referenced specifically in any publications.