Overview

  • Product name

    Anti-ALX3 antibody
  • Description

    Rabbit polyclonal to ALX3
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of total ALX3 protein.
  • Tested applications

    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from internal of human ALX3.

  • Positive control

    • 293 cell extract and HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab64985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • Relevance

    ALX3 (aristaless-like homeobox 3) is a nuclear protein with a homeobox DNA-binding domain. It functions as a transcriptional regulator with a possible role in patterning of the mesoderm during development. Preferential methylation of the ALX3 promoter is associated with advanced-stage neuroblastoma tumors.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • ALX 3 antibody
    • ALX3 homeobox 3 antibody
    • Aristaless like homeobox 3 antibody
    • FND1 antibody
    • Homeobox protein aristaless like 3 antibody
    • MGC138212 antibody
    • MGC141988 antibody
    • Proline rich transcription factor ALX3 antibody
    see all

Images

  • All lanes : Anti-ALX3 antibody (ab64985) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

  • Immunofluorescence analysis of HeLa cells, using ab64985 at 1/500-1/1000 dilution. Right hand panel was also treated with immunising peptide.

References

This product has been referenced in:

  • García-Sanz P  et al. Embryonic defence mechanisms against glucose-dependent oxidative stress require enhanced expression of Alx3 to prevent malformations during diabetic pregnancy. Sci Rep 7:389 (2017). Read more (PubMed: 28341857) »
  • Mirasierra M & Vallejo M Glucose-dependent downregulation of glucagon gene expression mediated by selective interactions between ALX3 and PAX6 in mouse alpha cells. Diabetologia 59:766-75 (2016). Read more (PubMed: 26739814) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (Skin tissue)
Permeabilization
No
Specification
Skin tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted May 29 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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