Product nameAnti-ALX3 antibody
DescriptionRabbit polyclonal to ALX3
SpecificityDetects endogenous levels of total ALX3 protein.
Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide from internal of human ALX3.
- 293 cell extract and HeLa cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab64985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
|ICC/IF||1/500 - 1/1000.|
RelevanceALX3 (aristaless-like homeobox 3) is a nuclear protein with a homeobox DNA-binding domain. It functions as a transcriptional regulator with a possible role in patterning of the mesoderm during development. Preferential methylation of the ALX3 promoter is associated with advanced-stage neuroblastoma tumors.
- ALX 3 antibody
- ALX3 homeobox 3 antibody
- Aristaless like homeobox 3 antibody
All lanes : Anti-ALX3 antibody (ab64985) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of HeLa cells, using ab64985 at 1/500-1/1000 dilution. Right hand panel was also treated with immunising peptide.
This product has been referenced in:
- García-Sanz P et al. Embryonic defence mechanisms against glucose-dependent oxidative stress require enhanced expression of Alx3 to prevent malformations during diabetic pregnancy. Sci Rep 7:389 (2017). Read more (PubMed: 28341857) »
- Mirasierra M & Vallejo M Glucose-dependent downregulation of glucagon gene expression mediated by selective interactions between ALX3 and PAX6 in mouse alpha cells. Diabetologia 59:766-75 (2016). Read more (PubMed: 26739814) »