Overview

  • Product name
  • Description
    Rabbit polyclonal to AMACR
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Prostate carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab12498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/25 - 1/50. Perform heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 before commencing with IHC staining protocol. We suggest an incubation period of 30 minutes at room temperature.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
    • Pathway
      Lipid metabolism; bile acid biosynthesis.
      Lipid metabolism; fatty acid metabolism.
    • Involvement in disease
      Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) [MIM:614307]. AMACRD results in elevated plasma concentrations of pristanic acid C27-bile-acid intermediates. It can be associated with polyneuropathy, retinitis pigmentosa, epilepsy.
      Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4) [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
    • Sequence similarities
      Belongs to the CaiB/BaiF CoA-transferase family.
    • Cellular localization
      Peroxisome. Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2 arylpropionyl CoA epimerase antibody
      • 2 methylacyl CoA racemase antibody
      • 2-methylacyl-CoA racemase antibody
      • Alpha methylacyl CoA racemase antibody
      • Alpha methylacyl Coenzyme A racemase antibody
      • Alpha methylacyl-CoA racemase deficiency, included antibody
      • Alpha-methylacyl-CoA racemase antibody
      • Amacr antibody
      • AMACR deficiency, included antibody
      • AMACR_HUMAN antibody
      • CBAS4 antibody
      • Da1-8 antibody
      • EC 5.1.99.4 antibody
      • Macr1 antibody
      • Methylacyl CoA racemase alpha antibody
      • RACE antibody
      • RM antibody
      see all

    Images

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue, staining AMACR with ab12498. Staining was detected using DAB.

    References

    ab12498 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    All of the antibodies you have chosen are appropriate for the experiments you are hoping to perform. All of the antibodies have been raised against the human protein but the cross reactivity with the mouse protein has not been ascertained and I cannot therefore say whether they will also be able to detect the mouse form of the protein.

    There are a few antibodies which may be worth considering as they may provide more experiments for the cost. All antibodies require optimisation before achieving the optimum results and it may therefore be that you will need to use more or less antibody than advised on the datasheets. The dilutions stated on the datasheets are guidelines only and will depend on a number of different factors.

    For the anti-AMACR antibody you may want to consider ab63340. This is amouse monoclonal, provided at a volume of 100 ul at the same price as ab12498 but has been shown to be usable at a dilution of1/500 to 1/2000 compared to 1/25 to 1/50 for ab12498 (500 uL provided).

    For the anti-Androgen Receptor antibody you may want to consider ab9474 (250 uL with a recommended dilution of 1/25-1/50). This should provide roughly the same number of experiments as ab47569 (100 uL with a recommended dilution of 1/50-1/100).

    For the anti-Mitochondria antibody, you may want to consider ab3298. This is provided at a volume of 500 uL with a recommended dilution of 1/500-1/1000 compared to ab92824 for which 100 uL is provided at a recommended dilution of 1/1000.

    For the anti-Prostate Specific Antigen antibody I was unable to identify another antibody which would provide you with more experiments.

    I hope this information has been of help. If you have any further questions please do not hesitate to contact us again.

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    Answer

    Thank you for your enquiry. Rabbit polyclonal to AMACR (ab12498) is suspended in PBS, with 0.05% sodium azide and BSA. The concentration is 100 ug/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for your enquiry. To address your questions: Please excuse my ignorance but according to the literature it seems that P504S and AMACR are interchangeable and synonyms of one another. From the details that I have I can tell you that this antibody was raised against full length P504S. I am in touch with the source of this antibody to determine the buffer components and the concentration of this antibody. I appreciate your patience in this matter. I will be in touch shortly.

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    Answer

    The immunogen used is a recombinant protein encoding full length P504S. If you have any further questions, please contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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