Overview

  • Product name

  • Description

    Rabbit polyclonal to AMBRA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human AMBRA1 aa 200-300 (internal sequence). The exact sequence is proprietary.
    Database link: Q9C0C7

  • Positive control

    • WB: Rat brain lysate. ICC/IF: SK-N-BE cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 143 kDa.
ICC/IF 1/100.

Target

  • Function

    Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
  • Sequence similarities

    Contains 3 WD repeats.
  • Cellular localization

    Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activating molecule in beclin 1 regulated autophagy antibody
    • Activating molecule in BECN1 regulated autophagy protein 1 antibody
    • Activating molecule in BECN1-regulated autophagy protein 1 antibody
    • Ambra1 antibody
    • AMRA1_HUMAN antibody
    • Autophagy and beclin 1 regulator 1 antibody
    • DCAF3 antibody
    • FLJ20294 antibody
    • Hypothetical protein FLJ20294 antibody
    • Hypothetical protein LOC55626 antibody
    • KIAA1736 antibody
    • MGC33725 antibody
    • WDR94 antibody
    see all

Images

  • 4% formaldehyde fixed SK-N-BE cells stained for AMBRA1 (green) using ab223351 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223351 (D) Merge.

  • Anti-AMBRA1 antibody (ab223351) at 1/1000 dilution + Rat brain lysate at 15 µg

    Secondary
    Goat Anti-Rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 143 kDa


    Exposure time: 6 minutes


    Blocking: 5% skimmed milk in 1X TBST.

References

ab223351 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223351.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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