Product nameAnti-AMBRA1 antibody
See all AMBRA1 primary antibodies
DescriptionRabbit polyclonal to AMBRA1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human AMBRA1 aa 200-300 (internal sequence). The exact sequence is proprietary.
Database link: Q9C0C7
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 143 kDa.|
FunctionRegulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
Sequence similaritiesContains 3 WD repeats.
Cellular localizationCytoplasmic vesicle > autophagosome.
- Information by UniProt
- Activating molecule in beclin 1 regulated autophagy antibody
- Activating molecule in BECN1 regulated autophagy protein 1 antibody
- Activating molecule in BECN1-regulated autophagy protein 1 antibody
4% formaldehyde fixed SK-N-BE cells stained for AMBRA1 (green) using ab223351 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/200 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223351 (D) Merge.
Anti-AMBRA1 antibody (ab223351) at 1/1000 dilution + Rat brain lysate at 15 µg
Goat Anti-Rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 6 minutes
Blocking: 5% skimmed milk in 1X TBST.
ab223351 has not yet been referenced specifically in any publications.