Key features and details
- Rabbit polyclonal to AMBRA1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-AMBRA1 antibody
See all AMBRA1 primary antibodies
DescriptionRabbit polyclonal to AMBRA1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human AMBRA1 (C terminal). AMBRA1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human AMBRA1 .
Database link: Q9C0C7
- WB: 3T3 (NIH) cell lysate. ICC/IF: K-562 cells and human brain cells. IHC-P: Human brain tissue
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).|
FunctionRegulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
Sequence similaritiesContains 3 WD repeats.
Cellular localizationCytoplasmic vesicle > autophagosome.
- Information by UniProt
- Activating molecule in beclin 1 regulated autophagy antibody
- Activating molecule in BECN1 regulated autophagy protein 1 antibody
- Activating molecule in BECN1-regulated autophagy protein 1 antibody
Anti-AMBRA1 antibody (ab69501) at 1 µg/ml + 3T3 cell lysate at 15 µg
Predicted band size: 143 kDa
Observed band size: 143 kDa
ab69501 at 5 µg/ml staining Ambra1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Immunofluorescence of Ambra1 in Human Brain cells using ab69501 at 20 ug/ml.
Immunocytochemistry/ Immunofluorescence analysis of K-562 cells labeling AMBRA1 with ab69501 at 20 μg/mL.
ab69501 has not yet been referenced specifically in any publications.