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Anti-AMBRA1 antibody (ab69503)

  • Datasheet
  • SDS
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Western blot - Anti-AMBRA1 antibody (ab69503)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMBRA1 antibody (ab69503)
  • Immunocytochemistry/ Immunofluorescence - Anti-AMBRA1 antibody (ab69503)

Key features and details

  • Rabbit polyclonal to AMBRA1
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-AMBRA1 antibody
    See all AMBRA1 primary antibodies
  • Description

    Rabbit polyclonal to AMBRA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    An 18 amino acid peptide from near the amino terminus of human AMBRA1

  • Positive control

    • Mouse brain tissue lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cardiovascular
    • Heart
    • Autophagy
    • Other
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab69503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 143 kDa.
Notes
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 143 kDa.

Target

  • Function

    Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
  • Sequence similarities

    Contains 3 WD repeats.
  • Cellular localization

    Cytoplasmic vesicle > autophagosome.
  • Target information above from: UniProt accession Q9C0C7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 55626 Human
    • Entrez Gene: 228361 Mouse
    • Omim: 611359 Human
    • SwissProt: Q9C0C7 Human
    • SwissProt: A2AH22 Mouse
    • Unigene: 654644 Human
    • Unigene: 436667 Mouse
    • Alternative names

      • Activating molecule in beclin 1 regulated autophagy antibody
      • Activating molecule in BECN1 regulated autophagy protein 1 antibody
      • Activating molecule in BECN1-regulated autophagy protein 1 antibody
      • Ambra1 antibody
      • AMRA1_HUMAN antibody
      • Autophagy and beclin 1 regulator 1 antibody
      • DCAF3 antibody
      • FLJ20294 antibody
      • Hypothetical protein FLJ20294 antibody
      • Hypothetical protein LOC55626 antibody
      • KIAA1736 antibody
      • MGC33725 antibody
      • WDR94 antibody
      see all

    Images

    • Western blot - Anti-AMBRA1 antibody (ab69503)
      Western blot - Anti-AMBRA1 antibody (ab69503)
      Anti-AMBRA1 antibody (ab69503) at 2 µg/ml + mouse brain tissue lysate at 15 µg

      Secondary
      Anti-rabbit

      Predicted band size: 143 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMBRA1 antibody (ab69503)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMBRA1 antibody (ab69503)

      ab69503 at 5 µg/ml staining AMBRA1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

    • Immunocytochemistry/ Immunofluorescence - Anti-AMBRA1 antibody (ab69503)
      Immunocytochemistry/ Immunofluorescence - Anti-AMBRA1 antibody (ab69503)
      Immunofluorescence of Ambra1 in Human Brain cells using ab69503 at 20 ug/ml.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab69503? Please let us know so that we can cite the reference in this datasheet.

    ab69503 has not yet been referenced specifically in any publications.

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