Anti-AMBRA1 antibody (ab69503)
Key features and details
- Rabbit polyclonal to AMBRA1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AMBRA1 antibody
See all AMBRA1 primary antibodies -
Description
Rabbit polyclonal to AMBRA1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
An 18 amino acid peptide from near the amino terminus of human AMBRA1
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Positive control
- Mouse brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 143 kDa.
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| Notes |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 143 kDa. |
Target
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Function
Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. -
Sequence similarities
Contains 3 WD repeats. -
Cellular localization
Cytoplasmic vesicle > autophagosome. - Information by UniProt
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Database links
- Entrez Gene: 55626 Human
- Entrez Gene: 228361 Mouse
- Omim: 611359 Human
- SwissProt: Q9C0C7 Human
- SwissProt: A2AH22 Mouse
- Unigene: 654644 Human
- Unigene: 436667 Mouse
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Alternative names
- Activating molecule in beclin 1 regulated autophagy antibody
- Activating molecule in BECN1 regulated autophagy protein 1 antibody
- Activating molecule in BECN1-regulated autophagy protein 1 antibody
see all
Images
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Western blot - Anti-AMBRA1 antibody (ab69503)Anti-AMBRA1 antibody (ab69503) at 2 µg/ml + mouse brain tissue lysate at 15 µg
Secondary
Anti-rabbit
Predicted band size: 143 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMBRA1 antibody (ab69503)ab69503 at 5 µg/ml staining AMBRA1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
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Immunocytochemistry/ Immunofluorescence - Anti-AMBRA1 antibody (ab69503)Immunofluorescence of Ambra1 in Human Brain cells using ab69503 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab69503 has not yet been referenced specifically in any publications.