Overview

  • Product name

    Anti-AMCase antibody [EPR19984]
    See all AMCase primary antibodies
  • Description

    Rabbit monoclonal [EPR19984] to AMCase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment within Human AMCase aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9BZP6

  • Positive control

    • WB: Mouse stomach and salivary gland lysates. IHC-P: Mouse stomach and lung tissues. IHC-Fr: Mouse stomach tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab207169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Use ab93684 as antigen retrieval buffer.

IHC-Fr 1/500.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

Target

  • Function

    Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.
  • Tissue specificity

    Detected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
    Contains 1 chitin-binding type-2 domain.
  • Cellular localization

    Cytoplasm and Secreted. Secretion depends on EGFR activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acidic mammalian chitinase [Precursor] antibody
    • Acidic mammalian chitinase antibody
    • AMCase antibody
    • Chia antibody
    • CHIA_HUMAN antibody
    • CHIT2 antibody
    • chitinase, acidic antibody
    • DKFZp313J1722 antibody
    • ECF L antibody
    • Lung specific protein TSA1902 antibody
    • Lung-specific protein TSA1902 antibody
    • RP5 1125M8.1 antibody
    • TSA1902 antibody
    see all

Images

  • Lane 1 : Anti-AMCase antibody [EPR19984] (ab207169) at 1/5000 dilution
    Lane 2 : Anti-AMCase antibody [EPR19984] (ab207169) at 1/2000 dilution

    Lane 1 : Mouse stomach lysate
    Lane 2 : Mouse liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 5 seconds; Lane 2: 30 seconds.

    Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon [PMID: 11085997; PMID: 12971947].

    Negative control: Mouse liver

  • Anti-AMCase antibody [EPR19984] (ab207169) at 1/2000 dilution + Mouse salivary gland lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Mouse AMCase mRNA is predominantly found in stomach and salivary gland, but not found in small intestine, liver and colon [PMID: 11085997; PMID: 12971947].

  • Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Strong positive staining on bottom gastric glands of mouse stomach is observed [PMID: 12971947].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on alveolar epithelium cells of mouse lung is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AMCase with ab207169 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse liver. [PMID:11085997]

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse stomach tissue labeling AMCase with ab207169 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Strong cytoplasm staining on bottom gastric glands of mouse stomach is observed [PMID: 12971947].

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab207169 has not yet been referenced specifically in any publications.

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