Key features and details
- Rabbit polyclonal to AMD1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-AMD1 antibody
See all AMD1 primary antibodies
DescriptionRabbit polyclonal to AMD1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human AMD1 (C terminal).
Database link: P17707
- WB: Rat kidney tissue lysate, Hela whole cell lysate. IHC-P: Human mammary cancer tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.|
PathwayAmine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.
Sequence similaritiesBelongs to the eukaryotic AdoMetDC family.
modificationsIs synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
- Information by UniProt
- Adenosylmethionine decarboxylase 1 antibody
- AdoMetDC antibody
- AMD1 antibody
Paraffin embedded human mammary cancer tissue stained for AMD1 using ab65820 at 1 µg/ml in immunohistochemical analysis.
All lanes : Anti-AMD1 antibody (ab65820) at 0.5 µg/ml
Lane 1 : Rat kidney tissue lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 40 µg
Predicted band size: 38 kDa
ICC/IF image of ab65820 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65820 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab65820 has been referenced in 1 publication.
- Dos Santos Ramos R et al. Regulation of the polyamine metabolic pathway in the endometrium of cows during early diestrus. Mol Reprod Dev N/A:N/A (2014). Cow . PubMed: 24659573