Overview

  • Product name

    Anti-Amelotin antibody
  • Description

    Rabbit polyclonal to Amelotin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 16-110 (SLPQLKPALG LPPTKLAPDQ GTLPNQQQSN QVFPSLSLIP LTQMLTLGPD LHLLNPAAGM TPGTQTHPLT LGGLNVQQQL HPHVLPIFVT QLGAQ) of Human Amelotin.

  • Positive control

    • Human cerebellum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122312 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122312, at 1/150, staining Amelotin in paraffin embedded Human cerebellum tissue by Immunohistochemistry.

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (follicle)
Specification
follicle
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 02 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (tooth)
Specification
tooth
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 03 2014

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