Overview

  • Product name

  • Description

    Rabbit polyclonal to AMF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AMF aa 508-558. The exact sequence is proprietary. NP_000166.2
    Database link: P06744

  • Positive control

    • IHC-P: Human ovarian carcinoma tissue. WB: HeLa, HEK-293T and Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as Glucose 6 phosphate isomerase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • Pathway

    Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
  • Involvement in disease

    Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • Sequence similarities

    Belongs to the GPI family.
  • Post-translational
    modifications

    Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.
  • Cellular localization

    Cytoplasm. Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMF antibody
    • Aurocrine motility factor antibody
    • Autocrine motility factor antibody
    • DKFZp686C13233 antibody
    • EC 5.3.1.9 antibody
    • G6PI_HUMAN antibody
    • Glucose phosphate isomerase antibody
    • Glucose-6-phosphate isomerase antibody
    • GNPI antibody
    • GPI antibody
    • Gpi1 antibody
    • Hexose monophosphate isomerase antibody
    • Hexosephosphate isomerase antibody
    • Neuroleukin antibody
    • NLK antibody
    • Oxoisomerase antibody
    • PGI antibody
    • PHI antibody
    • Phosphoglucose isomerase antibody
    • Phosphohexomutase antibody
    • Phosphohexose isomerase antibody
    • Phosphosaccharomutase antibody
    • SA 36 antibody
    • SA-36 antibody
    • SA36 antibody
    • Sperm antigen 36 antibody
    see all

Images

  • All lanes : Anti-AMF antibody (ab226203) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Exposure time: 10 seconds
  • Formalin fixed, paraffin embedded human ovarian carcinoma tissue stained for AFM with ab226203 (1/5,000 dilution) in immunohistochemical analysis. DAB detection. Counterstain: hematoxylin (blue).

  • AFM was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226203 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226203 at 1  µg/ml.

    Lane 1: ab226203 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 seconds.

References

ab226203 has not yet been referenced specifically in any publications.

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