Key features and details
- Rabbit polyclonal to AMF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AMF antibody
See all AMF primary antibodies
DescriptionRabbit polyclonal to AMF
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Pig, Chimpanzee
Recombinant fragment within Human AMF (internal sequence). The exact sequence is proprietary.
Database link: P06744
- WB: Raji and NCI-H1299 whole cell lysate. IHC-P: NCI-N87 xenograft tissue. ICC/IF: HepG2 cells.
This product was previously labelled as Glucose 6 phosphate isomerase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 63 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionBesides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
PathwayCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
Involvement in diseaseDefects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Sequence similaritiesBelongs to the GPI family.
modificationsPhosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
- AMF antibody
- Aurocrine motility factor antibody
- Autocrine motility factor antibody
All lanes : Anti-AMF antibody (ab227472) at 1/10000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 63 kDa
Paraffin-embedded NCI-N87 xenograft tissue stained for AFM with ab227472 at 1/500 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for AFM (green) using ab227472 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227472 has not yet been referenced specifically in any publications.