Overview

  • Product name

    Anti-AMF antibody [EPR11663(B)]
    See all AMF primary antibodies
  • Description

    Rabbit monoclonal [EPR11663(B)] to AMF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human AMF.
    Database link: P06744

  • Positive control

    • HepG2, HeLa, HT29, and A549 cell lysates; Human prostatic hyperplasia and Human thyroid carcinoma tissues; HepG2 and A549 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Glucose 6 phosphate isomerase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 63 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
    • Pathway

      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
    • Involvement in disease

      Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
    • Sequence similarities

      Belongs to the GPI family.
    • Post-translational
      modifications

      Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
      ISGylated.
    • Cellular localization

      Cytoplasm. Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMF antibody
      • Aurocrine motility factor antibody
      • Autocrine motility factor antibody
      • DKFZp686C13233 antibody
      • EC 5.3.1.9 antibody
      • G6PI_HUMAN antibody
      • Glucose phosphate isomerase antibody
      • Glucose-6-phosphate isomerase antibody
      • GNPI antibody
      • GPI antibody
      • Gpi1 antibody
      • Hexose monophosphate isomerase antibody
      • Hexosephosphate isomerase antibody
      • Neuroleukin antibody
      • NLK antibody
      • Oxoisomerase antibody
      • PGI antibody
      • PHI antibody
      • Phosphoglucose isomerase antibody
      • Phosphohexomutase antibody
      • Phosphohexose isomerase antibody
      • Phosphosaccharomutase antibody
      • SA 36 antibody
      • SA-36 antibody
      • SA36 antibody
      • Sperm antigen 36 antibody
      see all

    Images

    • All lanes : Anti-AMF antibody [EPR11663(B)] (ab167394) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HT29 cell lysate
      Lane 4 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 63 kDa

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling AFM with purified ab167394 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • ab167394 showing +ve staining in Human normal brain.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab167394 showing +ve staining in Human normal pancreas.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab167394 showing +ve staining in Human ovarian carcinoma.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • ab167394 showing +ve staining in Human skeletal muscle.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunofluorescent analysis of HepG2 cells labeling AFM with ab167394 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue, labeling AFM with ab167394 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling AFM with ab167394 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow cytometric analysis of permeabilized A549 cells, labeling AFM with ab167394 at 1/10 dilution.

    References

    ab167394 has not yet been referenced specifically in any publications.

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