
Recombinant Anti-AMF antibody [EPR11663(B)] (ab167394)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-AMF antibody [EPR11663(B)]
See all AMF primary antibodies -
Description
Rabbit monoclonal [EPR11663(B)] to AMF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AMF.
Database link: P06744 -
Positive control
- HepG2, HeLa, HT29, and A549 cell lysates; Human prostatic hyperplasia and Human thyroid carcinoma tissues; HepG2 and A549 cells.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Glucose 6 phosphate isomerase
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11663(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-AMF antibody [EPR11663(B)] (Alexa Fluor® 647) (ab203975)
- Anti-AMF antibody [EPR11663(B)] (Alexa Fluor® 488) (ab204382)
- Anti-AMF antibody [EPR11663(B)] (Phycoerythrin) (ab210599)
- Anti-AMF antibody [EPR11663(B)] (Alexa Fluor® 568) (ab213349)
- Anti-AMF antibody [EPR11663(B)] - BSA and Azide free (ab231691)
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab167394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 63 kDa. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | 1/100 - 1/500. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. -
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. -
Involvement in disease
Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. -
Sequence similarities
Belongs to the GPI family. -
Post-translational
modificationsPhosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
ISGylated. -
Cellular localization
Cytoplasm. Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 2821 Human
- Omim: 172400 Human
- SwissProt: P06744 Human
- Unigene: 466471 Human
-
Alternative names
- AMF antibody
- Aurocrine motility factor antibody
- Autocrine motility factor antibody
see all
Images
-
All lanes : Anti-AMF antibody [EPR11663(B)] (ab167394) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT29 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 63 kDa -
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling AFM with purified ab167394 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)
ab167394 showing +ve staining in Human normal brain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)
ab167394 showing +ve staining in Human normal pancreas.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)
ab167394 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)
ab167394 showing +ve staining in Human skeletal muscle.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunofluorescent analysis of HepG2 cells labeling AFM with ab167394 at 1/100 dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue, labeling AFM with ab167394 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (ab167394)
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling AFM with ab167394 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Flow cytometric analysis of permeabilized A549 cells, labeling AFM with ab167394 at 1/10 dilution.
Protocols
Datasheets and documents
References
ab167394 has not yet been referenced specifically in any publications.