Recombinant Anti-AMF antibody [EPR11663(B)] - BSA and Azide free (ab231691)


  • Product name

    Anti-AMF antibody [EPR11663(B)] - BSA and Azide free
    See all AMF primary antibodies
  • Description

    Rabbit monoclonal [EPR11663(B)] to AMF - BSA and Azide free
  • Host species

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human AMF. The exact sequence is proprietary.
    Database link: P06744

  • Positive control

    • HepG2, HeLa, HT29, and A549 cell lysates; Human prostatic hyperplasia and Human thyroid carcinoma tissues; HepG2 and A549 cells.
  • General notes

    Ab231691 is the carrier-free version of ab167394. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab231691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
    • Pathway

      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
    • Involvement in disease

      Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
    • Sequence similarities

      Belongs to the GPI family.
    • Post-translational

      Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    • Cellular localization

      Cytoplasm. Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMF antibody
      • Aurocrine motility factor antibody
      • Autocrine motility factor antibody
      • DKFZp686C13233 antibody
      • EC antibody
      • G6PI_HUMAN antibody
      • Glucose phosphate isomerase antibody
      • Glucose-6-phosphate isomerase antibody
      • GNPI antibody
      • GPI antibody
      • Gpi1 antibody
      • Hexose monophosphate isomerase antibody
      • Hexosephosphate isomerase antibody
      • Neuroleukin antibody
      • NLK antibody
      • Oxoisomerase antibody
      • PGI antibody
      • PHI antibody
      • Phosphoglucose isomerase antibody
      • Phosphohexomutase antibody
      • Phosphohexose isomerase antibody
      • Phosphosaccharomutase antibody
      • SA 36 antibody
      • SA-36 antibody
      • SA36 antibody
      • Sperm antigen 36 antibody
      see all


    ab231691 has not yet been referenced specifically in any publications.

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