Overview

  • Product name

    Anti-AMF antibody - N-terminal
    See all AMF primary antibodies
  • Description

    Rabbit polyclonal to AMF - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human AMF aa 1-50 (N terminal). The exact sequence is proprietary. NP_000166.2
    Database link: P06744

  • Positive control

    • IHC-P: Human lung carcinoma tissue. IP: HeLa whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as Glucose 6 phosphate isomerase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
    • Pathway

      Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
    • Involvement in disease

      Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
    • Sequence similarities

      Belongs to the GPI family.
    • Post-translational
      modifications

      Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
      ISGylated.
    • Cellular localization

      Cytoplasm. Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • AMF antibody
      • Aurocrine motility factor antibody
      • Autocrine motility factor antibody
      • DKFZp686C13233 antibody
      • EC 5.3.1.9 antibody
      • G6PI_HUMAN antibody
      • Glucose phosphate isomerase antibody
      • Glucose-6-phosphate isomerase antibody
      • GNPI antibody
      • GPI antibody
      • Gpi1 antibody
      • Hexose monophosphate isomerase antibody
      • Hexosephosphate isomerase antibody
      • Neuroleukin antibody
      • NLK antibody
      • Oxoisomerase antibody
      • PGI antibody
      • PHI antibody
      • Phosphoglucose isomerase antibody
      • Phosphohexomutase antibody
      • Phosphohexose isomerase antibody
      • Phosphosaccharomutase antibody
      • SA 36 antibody
      • SA-36 antibody
      • SA36 antibody
      • Sperm antigen 36 antibody
      see all

    Images

    • All lanes : Anti-AMF antibody - N-terminal (ab226212) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
      Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
      Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Exposure time: 10 seconds
    • Formalin fixed, paraffin embedded human lung carcinoma tissue stained for AFM with ab226212 (1/1000 dilution) in immunohistochemical analysis. DAB detection. Counterstain: hematoxylin (blue).

    • AFM was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226212 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226212 at 1 µg/ml.

      Lane 1: ab226212 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 3 seconds.

    References

    ab226212 has not yet been referenced specifically in any publications.

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