Product nameAnti-AMH antibody
See all AMH primary antibodies
DescriptionRabbit polyclonal to AMH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Horse, Guinea pig, Cow, Dog, Pig
- Human fetal small intestine lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab84952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceAnti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
- Anti muellerian hormone antibody
- MIF antibody
- MIS antibody
Anti-AMH antibody (ab84952) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal small intestine lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Gel concentration: 12%
ab84952 staining AMH in human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ab84952 has been referenced in 2 publications.
- Gowkielewicz M et al. Anti-Müllerian Hormone Expression in Endometrial Cancer Tissue. Int J Mol Sci 20:N/A (2019). PubMed: 30884769
- Nistal M et al. Sertoli cell dedifferentiation in human cryptorchidism and gender reassignment shows similarities between fetal environmental and adult medical treatment estrogen and antiandrogen exposure. Reprod Toxicol 42:172-9 (2013). Human . PubMed: 24012888