Overview

  • Product name

    Anti-AMH antibody - C-terminal
    See all AMH primary antibodies
  • Description

    Rabbit polyclonal to AMH - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Cow, Dog, Pig
  • Immunogen

    Recombinant fragment within Human AMH (C terminal). The exact sequence is proprietary.
    Database link: P03971

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Mouse ovary and testis tissues. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab229212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-AMH antibody - C-terminal (ab229212) at 1/2500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 59 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse ovary tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse testis tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.

  • Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for AMH (green) using ab229212 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229212 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229212.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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