Product nameAnti-AMH antibody - C-terminal
See all AMH primary antibodies
DescriptionRabbit polyclonal to AMH - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Cow, Dog, Pig
Recombinant fragment within Human AMH (C terminal). The exact sequence is proprietary.
Database link: P03971
- WB: HEK-293T, A431, HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Mouse ovary and testis tissues. ICC/IF: MCF7 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 59 kDa.|
|ICC/IF||1/100 - 1/1000.|
RelevanceAnti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.
- Anti muellerian hormone antibody
- MIF antibody
- MIS antibody
All lanes : Anti-AMH antibody - C-terminal (ab229212) at 1/2500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 59 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded mouse ovary tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse testis tissue stained for AMH using ab229212 at 1/500 dilution in immunohistochemical analysis.
Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for AMH (green) using ab229212 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
ab229212 has not yet been referenced specifically in any publications.