Overview

  • Product name

    Anti-AMHR2 antibody [MIG7]
    See all AMHR2 primary antibodies
  • Description

    Mouse monoclonal [MIG7] to AMHR2
  • Host species

    Mouse
  • Specificity

    Recognizes human cells bearing AMHR2 extracellular domain.
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, Indirect ELISAmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide:

    NANYSHLPPPGSPG

    , corresponding to amino acids 117-130 of Human AMHR2

Properties

Applications

Our Abpromise guarantee covers the use of ab64762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
Indirect ELISA Use at an assay dependent concentration.

Target

  • Function

    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone.
  • Involvement in disease

    Defects in AMHR2 are the cause of persistent Muellerian duct syndrome type 2 (PMDS2) [MIM:261550]. PMDS2 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMH type II receptor antibody
    • AMHR antibody
    • AMHR2 antibody
    • AMHR2_HUMAN antibody
    • Anti Mullerian hormone receptor, type II antibody
    • Anti-Muellerian hormone type II receptor antibody
    • Anti-Muellerian hormone type-2 receptor antibody
    • MGC141312 antibody
    • MIS type II receptor antibody
    • MISR2 antibody
    • MISRII antibody
    • MRII antibody
    • Muellerian hormone type 2 receptor antibody
    • Muellerian hormone type II receptor antibody
    • Muellerian inhibiting substance type II receptor antibody
    • Mullerian hormone receptor type II antibody
    • Mullerian inhibiting substance type II receptor antibody
    see all

Images

  • Overlay histogram showing K562 cells stained with ab64762 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64762, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:

  • Taximaimaiti R  et al. Expression of AMHR2 and C-KIT in cervical lesions in Uyghur Women of Xinjiang, China. Medicine (Baltimore) 97:e10793 (2018). Read more (PubMed: 29851788) »
  • Guven S  et al. Functional maintenance of differentiated embryoid bodies in microfluidic systems: a platform for personalized medicine. Stem Cells Transl Med 4:261-8 (2015). Read more (PubMed: 25666845) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

We do not have data from tests of bovine samples but BLAST of the immunogen sequence that generated the antibody shows 93% identity with the bovine protein. So, reactivity is likely, though not covered by our standard Abpromise guarantee of a replacement or refund in the event of failure.

Also, we do not have data from sorting live cells, only flow cytometry of cells fixed with methanol, but I expect the antibody is capable of binding to the extracellular domain without fixation.

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Question
Answer

Merci de nous avoir contactés.

J'ai contacté notre laboratoire qui m’a confirmé que ICC / IF n’a pas été testé pour ab64762. Je vous remercie d'avoir attiré notre attention à cette erreur car cela va nous permettre de mettre à jour notre fiche technique. Par conséquent, nous ne pouvons pas garantir que cet anticorps fonctionnera dans cette application. Malheureusement, nous n'avons pas d'autres anticorps dans notre catalogue qui soit garantis de fonctionner dans cette application.

Cependant, si vous souhaitez tester cet anticorps en ICC/IF, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, un avoir de la valeur de l'anticorps testé vous sera offert.

Étapes à suivre :

1. Nous informer que vous souhaitez tester un de nos anticorps en ICC/IF et nous vous enverrons un code promotionnel (valable 4 mois) à utiliser après obtention et envoi de vos résultats,

2. Acheter l'anticorps,

3. Nous envoyer vos résultats (positifs ou négatifs) obtenus sous forme d'Abreview (www.abcam.com/abreviews).

4. L'envoi de vos résultats activera le code et vous donnera un avoir de la valeur de l'anticorps testé.

J'espère que ces informations vous aideront. N'hésitez pas à me contacter si vous avez besoin de plus de renseignements.

J’ai aussi attaché le protocole que l’on recommande pour ICC/IF en pièce jointe.

Je vous suggère d’utiliser une dilution de 1/500 pour l’anticorps.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Monoclonal de lapin gratuit pour tout achat d'anticorps primaire, dans la limite des stocks disponibles. Notez le code "RABMAB-XBSMG" sur votre prochaine commande d’anticorps primaire. Pour plus d'informations, visitez le lien suivant: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for your inquiry.

I can confirm thatAnti-Müllerian Hormone Receptor is expressed by ovarian, breast, and prostate cancers.

Masiakos PT, et al. Human ovarian cancer, cell lines, and primary ascites cells express the human Müllerian inhibiting substance (MIS) Type II Receptor, bind, and are responsive to MIS. Clin Cancer Res 1999;5:3488-99

I can recommend that your customer also searches the literature for a suitable positive control in their experimental settings.

I hope this information is helpful. Please do not hesitate to contact me again with any further questions in this matter.

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Question
Answer

Thank you for contacting Abcam regarding ab64762. I have contacted the lab and we have not determined the cross-reactivity of this antibody with TGF beta. I am sorry for the inconvenience. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Endometrium)
Specification
Endometrium
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
No blocking step used for 15 minute(s) · Temperature: 25°C

Mr. Louie Ye

Verified customer

Submitted Sep 17 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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