Anti-AMID antibody - N-terminal (ab155326)

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Western blot - Anti-AMID antibody - N-terminal (ab155326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMID antibody - N-terminal (ab155326)

Key features and details

  • Rabbit polyclonal to AMID - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-AMID antibody - N-terminal
    See all AMID primary antibodies

  • Description

    Rabbit polyclonal to AMID - N-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1-43 of Human AMID (UniProt ID: Q9BRQ8).

  • Positive control

    • HepG2 and MOLT4 cell lysates; Human gastric carcinoma tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.

Target

  • Function

    Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Binds to DNA in a sequence-independent manner. May contribute to genotoxin-induced growth arrest.

  • Tissue specificity

    Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in heart, moderately in liver and skeletal muscles, and expressed at low levels in placenta, lung, kidney, and pancreas. Both transcripts expressed following p53/TP53 induction. The shorter 1.8 kb transcript seems to be the major transcript in EB1 colon cancer cells.

  • Sequence similarities

    Belongs to the FAD-dependent oxidoreductase family.

  • Cellular localization

    Cytoplasm. Mitochondrion outer membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • 5430437E11Rik antibody
      • aifm2 antibody
      • AIFM2_HUMAN antibody
      • AMID antibody
      • Apoptosis inducing factor (AIF) homologous mitochondrion associated inducer of death antibody
      • Apoptosis inducing factor (AIF) like mitochondrion associated inducer of death antibody
      • Apoptosis inducing factor mitochondrion associated 2 antibody
      • Apoptosis-inducing factor 2 antibody
      • Apoptosis-inducing factor homologous mitochondrion-associated inducer of death antibody
      • Apoptosis-inducing factor-like mitochondrion-associated inducer of death antibody
      • Cys51Stop antibody
      • HGNC11998 antibody
      • p53 responsive gene 3 antibody
      • p53 tumor suppressor antibody
      • p53-responsive gene 3 protein antibody
      • PRG3 antibody
      • TRP53 antibody
      • Tumor protein p53 antibody
      see all

    Images

    • Western blot - Anti-AMID antibody - N-terminal (ab155326)
      Western blot - Anti-AMID antibody - N-terminal (ab155326)
      Anti-AMID antibody - N-terminal (ab155326) at 1/1500 dilution + HepG2 cell lysate at 30 µg

      Predicted band size: 41 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMID antibody - N-terminal (ab155326)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMID antibody - N-terminal (ab155326)
      Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling AMID with ab155326 at 1/500 dilution.

    References (0)

    Publishing research using ab155326? Please let us know so that we can cite the reference in this datasheet.

    ab155326 has not yet been referenced specifically in any publications.

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