- References (1)
Product nameAmido black 10B, histological and protein dye
DescriptionHistological and protein dye.
- Acid Black 1
- Buffalo Black NBR
- Naphthol Blue Black
Diazo dye used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. Also utilized in cell proliferation assays and histochemical studies, and as a forensic stain typically used for enhancing latent prints contaminated with blood.
Chemical name4-Amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt
Storage instructionsStore at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
This product has been referenced in:
- Onder S et al. Decolorization of naphthol blue black using the horseradish peroxidase. Appl Biochem Biotechnol 163:433-43 (2011). Read more (PubMed: 20703828) »
- Tetlow LC & Woolley DE Histamine stimulates the proliferation of human articular chondrocytes in vitro and is expressed by chondrocytes in osteoarthritic cartilage. Ann Rheum Dis 62:991-4 (2003). Read more (PubMed: 12972479) »
- Perricone MA et al. Quantitation of fibroblast population growth rate in situ using computerized image analysis. Microsc Res Tech 31:257-64 (1995). Read more (PubMed: 7670164) »