Product nameAnti-AMIGO1 antibody [S86-36]
See all AMIGO1 primary antibodies
DescriptionMouse monoclonal [S86-36] to AMIGO1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Human AMIGO1 aa 554-574. (cytoplasmic C-terminus)
Database link: NP_065754.2
- WB: Rat brain membrane tissue lysate. ICC/IF: Neuroblastoma cell line SK-N-BE.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab167439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 60-80 kDa (predicted molecular weight: 55 kDa).|
FunctionPromotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain.
Sequence similaritiesBelongs to the immunoglobulin superfamily. AMIGO family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
DomainThe LRR repeat region mediates homodimerization.
Cellular localizationCell membrane. Cell projection > axon. Associated with axons of neuronal cells.
- Information by UniProt
- Adhesion molecule with Ig like domain 1 antibody
- ali2 antibody
- Alivin 2 antibody
Anti-AMIGO1 antibody [S86-36] (ab167439) at 1/200 dilution + Rat brain membrane tissue lysate at 15 µg
Goat Anti-Mouse IgG HRP at 1:1000 for 1 hour at RT
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 6 minutes
Block: 2% BSA and 2% Skim Milk in 1X TBST.
4% Formaldehyde-fixed SK-N-BE (Neuroblastoma cell line) cells stained for AMIGO1 (Green) using ab167439 at 1/100 dilution for 60 minutes at room temperature in ICC/IF analysis. Goat Anti-Mouse ATTO 488 was used as the secondary antibody at 1:100 for 60 min at RT. Phalloidin Texas Red F-Actin was used as a counterstain (red) at 1/1000 dilution for 60 minutes at room temperature. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at room temperature.
ab167439 has not yet been referenced specifically in any publications.