Key features and details
- Mouse monoclonal [S86-36] to AMIGO1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-AMIGO1 antibody [S86-36]
See all AMIGO1 primary antibodies
DescriptionMouse monoclonal [S86-36] to AMIGO1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Fusion protein corresponding to Human AMIGO1 aa 554-574. (cytoplasmic C-terminus)
Database link: NP_065754.2
- WB: Rat brain membrane tissue lysate. ICC/IF: Neuroblastoma cell line SK-N-BE.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab167439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 60-80 kDa (predicted molecular weight: 55 kDa).|
FunctionPromotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain.
Sequence similaritiesBelongs to the immunoglobulin superfamily. AMIGO family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
DomainThe LRR repeat region mediates homodimerization.
Cellular localizationCell membrane. Cell projection > axon. Associated with axons of neuronal cells.
- Information by UniProt
- Adhesion molecule with Ig like domain 1 antibody
- ali2 antibody
- Alivin 2 antibody
Anti-AMIGO1 antibody [S86-36] (ab167439) at 1/200 dilution + Rat brain membrane tissue lysate at 15 µg
Goat Anti-Mouse IgG HRP at 1:1000 for 1 hour at RT
Developed using the ECL technique.
Predicted band size: 55 kDa
Exposure time: 6 minutes
Block: 2% BSA and 2% Skim Milk in 1X TBST.
4% Formaldehyde-fixed SK-N-BE (Neuroblastoma cell line) cells stained for AMIGO1 (Green) using ab167439 at 1/100 dilution for 60 minutes at room temperature in ICC/IF analysis. Goat Anti-Mouse ATTO 488 was used as the secondary antibody at 1:100 for 60 min at RT. Phalloidin Texas Red F-Actin was used as a counterstain (red) at 1/1000 dilution for 60 minutes at room temperature. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab167439 has not yet been referenced specifically in any publications.