Overview

  • Product name

    Anti-AMIGO1 antibody [S86-36]
    See all AMIGO1 primary antibodies
  • Description

    Mouse monoclonal [S86-36] to AMIGO1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Human AMIGO1 aa 554-574. (cytoplasmic C-terminus)
    Database link: NP_065754.2

  • Positive control

    • WB: Rat brain membrane tissue lysate. ICC/IF: Neuroblastoma cell line SK-N-BE.

Properties

Applications

Our Abpromise guarantee covers the use of ab167439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60-80 kDa (predicted molecular weight: 55 kDa).
ICC/IF 1/100.

Target

  • Function

    Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. AMIGO family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 6 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
  • Domain

    The LRR repeat region mediates homodimerization.
  • Cellular localization

    Cell membrane. Cell projection > axon. Associated with axons of neuronal cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adhesion molecule with Ig like domain 1 antibody
    • ali2 antibody
    • Alivin 2 antibody
    • Alivin-2 antibody
    • AMGO1_HUMAN antibody
    • AMIGO 1 antibody
    • AMIGO antibody
    • AMIGO protein antibody
    • AMIGO-1 antibody
    • Amigo1 antibody
    • Amphoterin induced gene and ORF (Amigo) antibody
    • Amphoterin induced gene and ORF antibody
    • Amphoterin induced protein 1 antibody
    • Amphoterin-induced protein 1 antibody
    • KIAA1163 antibody
    • MGC25558 antibody
    • OTTHUMP00000013379 antibody
    • RP23 89M15.6 antibody
    see all

Images

  • Anti-AMIGO1 antibody [S86-36] (ab167439) at 1/200 dilution + Rat brain membrane tissue lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1:1000 for 1 hour at RT

    Developed using the ECL technique.

    Predicted band size: 55 kDa


    Exposure time: 6 minutes


    Block: 2% BSA and 2% Skim Milk in 1X TBST.

  • 4% Formaldehyde-fixed SK-N-BE (Neuroblastoma cell line) cells stained for AMIGO1 (Green) using ab167439 at 1/100 dilution for 60 minutes at room temperature in ICC/IF analysis. Goat Anti-Mouse ATTO 488 was used as the secondary antibody at 1:100 for 60 min at RT. Phalloidin Texas Red F-Actin was used as a counterstain (red) at 1/1000 dilution for 60 minutes at room temperature. The nuclear counterstain was DAPI (Blue) at 1/5000 dilution for 5 minutes at room temperature.

References

ab167439 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab167439.
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