Anti-Amino-terminal enhancer of split/AES antibody (ab118881)
Key features and details
- Rabbit polyclonal to Amino-terminal enhancer of split/AES
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Amino-terminal enhancer of split/AES antibody
See all Amino-terminal enhancer of split/AES primary antibodies -
Description
Rabbit polyclonal to Amino-terminal enhancer of split/AES -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Amino-terminal enhancer of split/AES (C terminal) conjugated to keyhole limpet haemocyanin.
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Positive control
- IHC-P: Human skeletal muscle. WB: HEK-293 cell lysate.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
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IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development. -
Tissue specificity
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver. -
Sequence similarities
Belongs to the WD repeat Groucho/TLE family. -
Domain
Lacks the C-terminal WD repeats. -
Post-translational
modificationsUbiquitinated by XIAP/BIRC4. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 166 Human
- Entrez Gene: 14797 Mouse
- Entrez Gene: 29466 Rat
- Omim: 600188 Human
- SwissProt: Q08117 Human
- SwissProt: P63002 Mouse
- SwissProt: P63003 Rat
- Unigene: 515053 Human
see all -
Alternative names
- Aes 1 antibody
- Aes 2 antibody
- aes antibody
see all
Images
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Human skeletal muscle (formalin fixed, paraffin embedded) stained with ab118881 at 2.5 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Lane 1 : Anti-Amino-terminal enhancer of split/AES antibody (ab118881) at 1 µg/ml
Lane 2 : Anti-Amino-terminal enhancer of split/AES antibody (ab118881) at 2 µg/ml
Lane 3 : Anti-Amino-terminal enhancer of split/AES antibody (ab118881) at 4 µg/ml
All lanes : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 21 kDa
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab118881 has not yet been referenced specifically in any publications.