Product nameAnti-Amino-terminal enhancer of split antibody
See all Amino-terminal enhancer of split primary antibodies
DescriptionRabbit polyclonal to Amino-terminal enhancer of split
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- This antibody gives a positive signal in WB against Recombinant His-tagged GRG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab16638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 22 kDa).|
FunctionTranscriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
Tissue specificityFound predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
Sequence similaritiesBelongs to the WD repeat Groucho/TLE family.
DomainLacks the C-terminal WD repeats.
modificationsUbiquitinated by XIAP/BIRC4.
- Information by UniProt
- Aes 1 antibody
- Aes 2 antibody
- aes antibody
All lanes : Anti-Amino-terminal enhancer of split antibody (ab16638) at 1 µg/ml
Lanes 6-10 : Human Amino-terminal enhancer of split peptide (ab16684) at 1 µg/ml
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible cross reactivity), 28 kDa (possible cross reactivity), 33 kDa (possible cross reactivity), 40 kDa (possible cross reactivity), 53 kDa (possible cross reactivity), 75 kDa (possible cross reactivity), 80 kDa (possible cross reactivity)
1 - 10 : GRG (Groucho homologue) antibody (ab16638) at 1 ug/ml
6 - 10 : GRG (Groucho homologue) peptide (ab16684) at 1 ug/ml
1, 6: Recombinant His-tagged GRG (28ug)
2, 7: Human muscle (20ug)
3, 8: Human heart (20ug)
4, 9: Human embryonic stem cell lysate
5, 10: Mouse embryonic stem cell lysate
Observed band size : 32 kDa (arrow)
Additional bands at : 21 kDa (possible non-specific binding, possible endogenous GRG), 28 kDa (possible non-specific binding, possible endogenous GRG), 35 kDa (possible non-specific binding), 40 kDa (possible non-specific binding), 53 kDa (possible non-specific binding), 75 kDa (possible non-specific binding), 80 kDa (possible non-specific binding).
A major band coresponding to GRG was observed in HEK293 lysates in which His-tagged GR had been overexpressed. Many
ab16638 has not yet been referenced specifically in any publications.