Anti-Amino-terminal enhancer of split antibody - N-terminal (ab166789)

Overview

  • Product name
    Anti-Amino-terminal enhancer of split antibody - N-terminal
    See all Amino-terminal enhancer of split primary antibodies
  • Description
    Goat polyclonal to Amino-terminal enhancer of split - N-terminal
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Cow, Dog, Gorilla, Opossum, Frog
  • Immunogen

    Synthetic peptide:

    SHLPQQLKFTTSD

    -C, corresponding to N terminal amino acids 12-24 of Human Amino-terminal enhancer of split (NP_001121.2).

  • Positive control
    • Human colon, submucosal plexus tissue; Human brain (cerebellum and cerebral cortex) lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab166789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21, 29 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
  • Tissue specificity
    Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
  • Sequence similarities
    Belongs to the WD repeat Groucho/TLE family.
  • Domain
    Lacks the C-terminal WD repeats.
  • Post-translational
    modifications
    Ubiquitinated by XIAP/BIRC4.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aes 1 antibody
    • Aes 2 antibody
    • aes antibody
    • AES_HUMAN antibody
    • Aes1 antibody
    • Aes2 antibody
    • Amino enhancer of split antibody
    • Amino terminal enhancer of split antibody
    • Amino-terminal enhancer of split antibody
    • Esp 1 antibody
    • Esp1 antibody
    • Gp130 associated protein GAM antibody
    • Gp130-associated protein GAM antibody
    • GRG 5 antibody
    • GRG antibody
    • GRG protein antibody
    • Grg-5 antibody
    • GRG5 antibody
    • Groucho-related protein 5 antibody
    • Protein ESP 1 antibody
    • Protein ESP1 antibody
    • Protein GRG antibody
    • TLE 5 antibody
    • TLE5 antibody
    see all

Images

  • Anti-Amino-terminal enhancer of split antibody - N-terminal (ab166789) at 0.3 µg/ml + Human cerebral cortex tissue lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 21, 29 kDa
    Observed band size: 30 kDa (why is the actual band size different from the predicted?)



    Primary incubation: 1 hour.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon, submucosal plexus tissue labeling Amino-terminal enhancer of split with ab166789 at 3.75µg/ml.

References

ab166789 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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