Anti-Amino-terminal enhancer of split/AES antibody (ab166789)
Key features and details
- Goat polyclonal to Amino-terminal enhancer of split/AES
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Amino-terminal enhancer of split/AES antibody
See all Amino-terminal enhancer of split/AES primary antibodies -
Description
Goat polyclonal to Amino-terminal enhancer of split/AES -
Host species
Goat -
Specificity
ab166789 is expected to recognize all reported isoforms of Amino-terminal enhancer of split/AES (NP_945320.1; NP_001121.2; NP_945321.1).
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Cow, Dog, Gorilla, Opossum, Frog
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Immunogen
Synthetic peptide corresponding to Human Amino-terminal enhancer of split/AES aa 12-24 (N terminal) (Cysteine residue).
Sequence:SHLPQQLKFTTSD
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Positive control
- Human colon, submucosal plexus tissue; Human brain (cerebellum and cerebral cortex) lysates.
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General notes
This product was previously labelled as Amino-terminal enhancer of split
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab166789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21, 29 kDa). | |
| IHC-P | Use a concentration of 3.75 µg/ml. |
Target
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Function
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development. -
Tissue specificity
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver. -
Sequence similarities
Belongs to the WD repeat Groucho/TLE family. -
Domain
Lacks the C-terminal WD repeats. -
Post-translational
modificationsUbiquitinated by XIAP/BIRC4. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 505375 Cow
- Entrez Gene: 485064 Dog
- Entrez Gene: 166 Human
- Entrez Gene: 14797 Mouse
- Entrez Gene: 29466 Rat
- Omim: 600188 Human
- SwissProt: Q08117 Human
- SwissProt: P63002 Mouse
see all -
Alternative names
- Aes 1 antibody
- Aes 2 antibody
- aes antibody
see all
Images
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Western blot - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)Anti-Amino-terminal enhancer of split/AES antibody (ab166789) at 0.3 µg/ml + Human cerebral cortex tissue lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 21, 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon, submucosal plexus tissue labeling Amino-terminal enhancer of split/AES with ab166789 at 3.75µg/ml.
Protocols
Datasheets and documents
References (0)
ab166789 has not yet been referenced specifically in any publications.
