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Anti-Amino-terminal enhancer of split/AES antibody (ab166789)

  • Datasheet
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Western blot - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)

Key features and details

  • Goat polyclonal to Amino-terminal enhancer of split/AES
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Amino-terminal enhancer of split/AES protein (ab104756)
Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-Amino-terminal enhancer of split/AES antibody
    See all Amino-terminal enhancer of split/AES primary antibodies
  • Description

    Goat polyclonal to Amino-terminal enhancer of split/AES
  • Host species

    Goat
  • Specificity

    ab166789 is expected to recognize all reported isoforms of Amino-terminal enhancer of split/AES (NP_945320.1; NP_001121.2; NP_945321.1).

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Cow, Dog, Gorilla, Opossum, Frog
  • Immunogen

    Synthetic peptide corresponding to Human Amino-terminal enhancer of split/AES aa 12-24 (N terminal) (Cysteine residue).
    Sequence:

    SHLPQQLKFTTSD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human colon, submucosal plexus tissue; Human brain (cerebellum and cerebral cortex) lysates.
  • General notes

     This product was previously labelled as Amino-terminal enhancer of split

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Amino-terminal enhancer of split/AES protein (ab104756)

Applications

Our Abpromise guarantee covers the use of ab166789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21, 29 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function

    Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
  • Tissue specificity

    Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
  • Sequence similarities

    Belongs to the WD repeat Groucho/TLE family.
  • Domain

    Lacks the C-terminal WD repeats.
  • Post-translational
    modifications

    Ubiquitinated by XIAP/BIRC4.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q08117 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 505375 Cow
    • Entrez Gene: 485064 Dog
    • Entrez Gene: 166 Human
    • Entrez Gene: 14797 Mouse
    • Entrez Gene: 29466 Rat
    • Omim: 600188 Human
    • SwissProt: Q08117 Human
    • SwissProt: P63002 Mouse
    • SwissProt: P63003 Rat
    • Unigene: 515053 Human
    • Unigene: 180013 Mouse
    • Unigene: 11495 Rat
    see all
  • Alternative names

    • Aes 1 antibody
    • Aes 2 antibody
    • aes antibody
    • AES_HUMAN antibody
    • Aes1 antibody
    • Aes2 antibody
    • Amino enhancer of split antibody
    • Amino terminal enhancer of split antibody
    • Amino-terminal enhancer of split antibody
    • Esp 1 antibody
    • Esp1 antibody
    • Gp130 associated protein GAM antibody
    • Gp130-associated protein GAM antibody
    • GRG 5 antibody
    • GRG antibody
    • GRG protein antibody
    • Grg-5 antibody
    • GRG5 antibody
    • Groucho-related protein 5 antibody
    • Protein ESP 1 antibody
    • Protein ESP1 antibody
    • Protein GRG antibody
    • TLE 5 antibody
    • TLE5 antibody
    see all

Images

  • Western blot - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)
    Western blot - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)
    Anti-Amino-terminal enhancer of split/AES antibody (ab166789) at 0.3 µg/ml + Human cerebral cortex tissue lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 21, 29 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Primary incubation: 1 hour.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon, submucosal plexus tissue labeling Amino-terminal enhancer of split/AES with ab166789 at 3.75&microg/ml.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab166789? Please let us know so that we can cite the reference in this datasheet.

    ab166789 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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