Anti-Amino-terminal enhancer of split/AES antibody (ab166789)
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Overview
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Product name
Anti-Amino-terminal enhancer of split/AES antibody
See all Amino-terminal enhancer of split/AES primary antibodies -
Description
Goat polyclonal to Amino-terminal enhancer of split/AES -
Host species
Goat -
Specificity
ab166789 is expected to recognize all reported isoforms of Amino-terminal enhancer of split/AES (NP_945320.1; NP_001121.2; NP_945321.1).
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster, Cow, Dog, Gorilla, Opossum, Frog
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Immunogen
Synthetic peptide corresponding to Human Amino-terminal enhancer of split/AES aa 12-24 (N terminal) (Cysteine residue).
Sequence:SHLPQQLKFTTSD
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Positive control
- Human colon, submucosal plexus tissue; Human brain (cerebellum and cerebral cortex) lysates.
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General notes
This product was previously labelled as Amino-terminal enhancer of split
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab166789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21, 29 kDa). | |
| IHC-P | Use a concentration of 3.75 µg/ml. |
Target
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Function
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development. -
Tissue specificity
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver. -
Sequence similarities
Belongs to the WD repeat Groucho/TLE family. -
Domain
Lacks the C-terminal WD repeats. -
Post-translational
modificationsUbiquitinated by XIAP/BIRC4. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 505375 Cow
- Entrez Gene: 485064 Dog
- Entrez Gene: 166 Human
- Entrez Gene: 14797 Mouse
- Entrez Gene: 29466 Rat
- Omim: 600188 Human
- SwissProt: Q08117 Human
- SwissProt: P63002 Mouse
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Alternative names
- Aes 1 antibody
- Aes 2 antibody
- aes antibody
see all
Images
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Western blot - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)Anti-Amino-terminal enhancer of split/AES antibody (ab166789) at 0.3 µg/ml + Human cerebral cortex tissue lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 21, 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Primary incubation: 1 hour. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amino-terminal enhancer of split/AES antibody (ab166789)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon, submucosal plexus tissue labeling Amino-terminal enhancer of split/AES with ab166789 at 3.75µg/ml.
Datasheets and documents
References
ab166789 has not yet been referenced specifically in any publications.
