Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-Amino-terminal enhancer of split/AES antibody [EPR8385]
See all Amino-terminal enhancer of split/AES primary antibodies
DescriptionRabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human Amino-terminal enhancer of split/AES aa 150-250 (C terminal).
Database link: Q08117
- 293T, HeLa, PC3 and Jurkat cell lysates; HeLa cells.
This product was previously labelled as Amino-terminal enhancer of split
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 21 kDa.|
|ICC/IF||1/250 - 1/500.|
FunctionTranscriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
Tissue specificityFound predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
Sequence similaritiesBelongs to the WD repeat Groucho/TLE family.
DomainLacks the C-terminal WD repeats.
modificationsUbiquitinated by XIAP/BIRC4.
- Information by UniProt
- Aes 1 antibody
- Aes 2 antibody
- aes antibody
All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC3 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 21 kDa
Immunofluorescent staining of HeLa cells labelling Amino-terminal enhancer of split/AES using ab137060 at 1/250 dilution.
ab137060 has not yet been referenced specifically in any publications.