Recombinant
RabMAb

Recombinant Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Amino-terminal enhancer of split/AES antibody [EPR8385]
    See all Amino-terminal enhancer of split/AES primary antibodies

  • Description

    Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human Amino-terminal enhancer of split/AES aa 150-250 (C terminal).
    Database link: Q08117

  • Positive control

    • 293T, HeLa, PC3 and Jurkat cell lysates; HeLa cells.

  • General notes

     

     

     This product was previously labelled as Amino-terminal enhancer of split

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab137060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.

    • Tissue specificity

      Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.

    • Sequence similarities

      Belongs to the WD repeat Groucho/TLE family.

    • Domain

      Lacks the C-terminal WD repeats.

    • Post-translational
      modifications

      Ubiquitinated by XIAP/BIRC4.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Aes 1 antibody
      • Aes 2 antibody
      • aes antibody
      • AES_HUMAN antibody
      • Aes1 antibody
      • Aes2 antibody
      • Amino enhancer of split antibody
      • Amino terminal enhancer of split antibody
      • Amino-terminal enhancer of split antibody
      • Esp 1 antibody
      • Esp1 antibody
      • Gp130 associated protein GAM antibody
      • Gp130-associated protein GAM antibody
      • GRG 5 antibody
      • GRG antibody
      • GRG protein antibody
      • Grg-5 antibody
      • GRG5 antibody
      • Groucho-related protein 5 antibody
      • Protein ESP 1 antibody
      • Protein ESP1 antibody
      • Protein GRG antibody
      • TLE 5 antibody
      • TLE5 antibody
      see all

    Images

    • Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 21 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

      Immunofluorescent staining of HeLa cells labelling Amino-terminal enhancer of split/AES using ab137060 at 1/250 dilution.

    References (0)

    Publishing research using ab137060? Please let us know so that we can cite the reference in this datasheet.

    ab137060 has not yet been referenced specifically in any publications.

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