Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

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Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
  • Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
  • Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
  • Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Amino-terminal enhancer of split/AES antibody [EPR8385]
    See all Amino-terminal enhancer of split/AES primary antibodies

  • Description

    Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human Amino-terminal enhancer of split/AES aa 150-250 (C terminal).
    Database link: Q08117

  • Positive control

    • WB: HCT116, 293T, HeLa, PC3 and Jurkat cell lysates. ICC: HeLa cells.

  • General notes

     

     

     This product was previously labelled as Amino-terminal enhancer of split

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab137060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.

    • Tissue specificity

      Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.

    • Sequence similarities

      Belongs to the WD repeat Groucho/TLE family.

    • Domain

      Lacks the C-terminal WD repeats.

    • Post-translational
      modifications

      Ubiquitinated by XIAP/BIRC4.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Aes 1 antibody
      • Aes 2 antibody
      • aes antibody
      • AES_HUMAN antibody
      • Aes1 antibody
      • Aes2 antibody
      • Amino enhancer of split antibody
      • Amino terminal enhancer of split antibody
      • Amino-terminal enhancer of split antibody
      • Esp 1 antibody
      • Esp1 antibody
      • Gp130 associated protein GAM antibody
      • Gp130-associated protein GAM antibody
      • GRG 5 antibody
      • GRG antibody
      • GRG protein antibody
      • Grg-5 antibody
      • GRG5 antibody
      • Groucho-related protein 5 antibody
      • Protein ESP 1 antibody
      • Protein ESP1 antibody
      • Protein GRG antibody
      • TLE 5 antibody
      • TLE5 antibody
      see all

    Images

    • Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution

      Lane 1 : Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
      Lane 2 : AES knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
      Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 4 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab137060 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab137060 Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] was shown to specifically react with Amino-terminal enhancer of split/AES in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266886 (knockout cell lysate ab257818) was used. Wild-type and Amino-terminal enhancer of split/AES knockout samples were subjected to SDS-PAGE. ab137060 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      Immunocytochemistry/ Immunofluorescence - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

      Immunofluorescent staining of HeLa cells labelling Amino-terminal enhancer of split/AES using ab137060 at 1/250 dilution.

    • Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      Western blot - Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 21 kDa

    • Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)
      Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

    References (0)

    Publishing research using ab137060? Please let us know so that we can cite the reference in this datasheet.

    ab137060 has not yet been referenced specifically in any publications.

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