Recombinant
RabMAb

Recombinant Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060)

Overview

  • Product name

    Anti-Amino-terminal enhancer of split/AES antibody [EPR8385]
    See all Amino-terminal enhancer of split/AES primary antibodies
  • Description

    Rabbit monoclonal [EPR8385] to Amino-terminal enhancer of split/AES
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Amino-terminal enhancer of split/AES aa 150-250 (C terminal).
    Database link: Q08117

  • Positive control

    • 293T, HeLa, PC3 and Jurkat cell lysates; HeLa cells.
  • General notes

     

     

     This product was previously labelled as Amino-terminal enhancer of split

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
    • Tissue specificity

      Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
    • Sequence similarities

      Belongs to the WD repeat Groucho/TLE family.
    • Domain

      Lacks the C-terminal WD repeats.
    • Post-translational
      modifications

      Ubiquitinated by XIAP/BIRC4.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Aes 1 antibody
      • Aes 2 antibody
      • aes antibody
      • AES_HUMAN antibody
      • Aes1 antibody
      • Aes2 antibody
      • Amino enhancer of split antibody
      • Amino terminal enhancer of split antibody
      • Amino-terminal enhancer of split antibody
      • Esp 1 antibody
      • Esp1 antibody
      • Gp130 associated protein GAM antibody
      • Gp130-associated protein GAM antibody
      • GRG 5 antibody
      • GRG antibody
      • GRG protein antibody
      • Grg-5 antibody
      • GRG5 antibody
      • Groucho-related protein 5 antibody
      • Protein ESP 1 antibody
      • Protein ESP1 antibody
      • Protein GRG antibody
      • TLE 5 antibody
      • TLE5 antibody
      see all

    Images

    • All lanes : Anti-Amino-terminal enhancer of split/AES antibody [EPR8385] (ab137060) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 21 kDa

    • Immunofluorescent staining of HeLa cells labelling Amino-terminal enhancer of split/AES using ab137060 at 1/250 dilution.

    References

    ab137060 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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