Aminohippuric acid Assay Kit (Colorimetric) (ab204725)

Overview

  • Product name

    Aminohippuric acid Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Plasma
  • Assay type

    Quantitative
  • Product overview

    Aminohippuric acid Assay Kit (ab204725) utilizes a 96-well format in which p-Aminohippuric acid (PAH) reacts with Dimethylaminocinnamaldehyde (DACA) which gives a strongly colored derivative at OD = 550 nm. The amounts of PAH in urine and plasma can easily be quantified with this product.

  • Notes

    p-Aminohippuric acid (PAH) is a derivative of hippuric acid and useful as a diagnostic agent for the measurement of renal plasma flow. About 20-30% is completely filtered by the glomerulus and not readsorbed by the tubules. The remainder which bypasses the glomerulus and enters the tubules is completely secreted. At low doses, PAH is almost completely removed with one-pass through the kidneys. Hence, the venous concentration of PAH is close to zero and PAH has been used to determine the effective renal plasma flow (eRPF) from the plasma. The venous concentration is usually <10% that of the plasma concentration so eRPF slightly underestimates the actual RPF. This error is generally accepted because of the ease with which PAH infusion allows calculation of eRPF:

    eRPF = ([PAH]U/[PAH]P) x Urine Volume

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    DACA solution 1 x 15ml
    PAH Standard (10 mg/ml) 1 x 100µl
    TCA (15 %) 1 x 15ml

Images

  • Typical PAH Standard calibration curve

Protocols

References

ab204725 has not yet been referenced specifically in any publications.

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