Product nameAmmonia Assay Kit
See all Ammonia kits
Sample typeUrine, Other biological fluids
Range0.012 mM - 1 mM
Ammonia Assay Kit (ab272538) is simple, direct and automation-ready procedures for measuring NH3 are very desirable. This assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 360/450nm) is proportional to the ammonia concentration in the sample.
Fast and sensitive: Linear detection range of 0.012 - 1 mM ammonia.
Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Tested applicationsSuitable for: Functional Studiesmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 200 tests Assay Buffer 1 x 20ml Reagent A 1 x 1ml Reagent B 1 x 1ml Standard (20mM Ammonium chloride) 1 x 400µl
RelevanceAmmonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.
Our Abpromise guarantee covers the use of ab272538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab272538 has not yet been referenced specifically in any publications.