Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509)

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Overview

  • Product name
    Ammonia Assay Kit - Modified Berthelot - (Colorimetric)
    See all Ammonia kits
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Cell culture media
  • Assay type
    Quantitative
  • Sensitivity
    < 10 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam's Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.

     

    Store the kit at +4°C.

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 100 tests
    Ammonia Reagent 1 Amber 1 x 8ml
    Ammonia Reagent 2 Clear 1 x 4ml
    Ammonium Chloride Standard Yellow 1 x 0.1ml
  • Research areas
  • Relevance
    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.

Images

  • Functional Studies - Ammonia Colorimetric Assay Kit (Modified Berthelot Assay) (ab102509)
    Functional Studies - Ammonia Colorimetric Assay Kit (Modified Berthelot Assay) (ab102509)

    Standard curve: mean of duplicates (+/- SD) with background reads subtracted.

  • Functional Studies - Ammonia Colorimetric Assay Kit (Modified Berthelot Assay) (ab102509)
    Functional Studies - Ammonia Colorimetric Assay Kit (Modified Berthelot Assay) (ab102509)

    Ammonia measured in biological fluids showing quantity (mM) in tested samples. Samples were diluted 10-1000 fold.

Protocols

References

ab102509 has not yet been referenced specifically in any publications.

Publishing research using ab102509? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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7 reviews or Q&A

10
Votes: Highest First

1. Is pH of the sample an important consideration when using this kit?
2. Can you recommend a protocol for preparing mouse stool samples for use with this kit?

Thank you for contacting us.
In response to your questions on this kit, the pH of the sample should be ˜7-8. To use mouse stool sample, add assay buffer to sample. Mix it completely either by pipetting up & down, sonicating or vortexing. Centr...

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Customer kindly contacted us regarding this product. In particular, they are wondering whether a deproteinizing step would be needed when using this on murine urine samples as protein levels in urine are generally low.

Thank you for contacting us. I have spoken with the lab regarding the need to deproteinize mouse urine samples with this kit. They would still strongly recommned this step to attain the best possible data with this product. I hope this informatio...

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I was wondering if this kit can be used to measure ammonium (instead of ammonia) and if so, is the procedure the same? What is the detection limit?   Thank you.

Thank you for contacting us.

This product may be used to measure ammonium. In thisassay, the standards used are ammonium. Adjustment of the protocol is not required and detection levels will be the same.


I hope this infor...

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Product code: 102509
Lot number:
Inquiry: Hello, I am trying to set up the ammonia chloride standard curve using the reagents from your kit (ab102509). I am doing the assay in a standard, clear 96-well plate the reagents at room temperature. ...

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Thank you for your inquiry.

The most common reason for a low standard curve is a mistake when preparing the dilutions. I can tell from my on personal experience that a 10 times calculation mistake is especially easy to make.

Therefo...

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How long can this product been stored after opening?

Thank you for calling Abcam earlier today

I have talked to the lab and the kit components are stable for at least 6 months (and up to 12 month) from the shipping date, if stored at the right temp and conditions.

Please let me know i...

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We are hoping to detect and quantify the following analytes (all human): Plasma glucose Plasma insulin Serum triglycerides Serum urea Serum creatinine Serum total protein Urinary urea Urinary ammonia At the moment I am trying to pick the most suitable ...

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Thank you for contacting us. The kits you listed are appropriate for your samples, though we recommend ab83360 over ab102509 for assay of ammonia in urine samples. None have been published, to our knowledge. For a quote, we will need a shippin...

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Will either of these kits work with serum samples? If so, do you have a sample preparation protocol?

Thank you for your inquiry. The lab confirmed that yes, these assay kits are compatible with serum samples from mammalian samples but they did not provide an exact protocol. I've included our general serum sample protocol below: For serum: Coll...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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