Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture media, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 10 µM
Product nameAmmonia Assay Kit - Modified Berthelot - (Colorimetric)
See all Ammonia kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Cell culture media
Sensitivity< 10 µM
Assay time0h 30m
Ammonia Assay Kit - Modified Berthelot (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay to measure ammonia.
In this non-enzymatic ammonia assay protocol, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.
Ammonia assay protocol summary:
- add samples and standards to wells
- add reagent 1 and reagent 2 and incubate for 30 min
- analyze with a microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K470 Ammonia Colorimetric Assay Kit II. K470-100 is the same size as the 100 test size of ab102509.
Ammonia assay kit ab83360 is an alternative to this product, and is the most popular ammonia assay kit.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 100 tests Ammonia Reagent 1 Amber 1 x 8ml Ammonia Reagent 2 Clear 1 x 4ml Ammonium Chloride Standard Yellow 1 x 0.1ml
RelevanceAmmonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.
ab102509 has been referenced in 2 publications.
- Raghavan SS et al. Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli. Microb Cell Fact 18:139 (2019). PubMed: 31426802
- Wu C et al. Fluxomic Analysis Reveals Central Carbon Metabolism Adaptation for Diazotroph Azotobacter vinelandii Ammonium Excretion. Sci Rep 9:13209 (2019). PubMed: 31520074