Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509)


  • Product name
    Ammonia Assay Kit - Modified Berthelot - (Colorimetric)
    See all Ammonia kits
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Cell culture media
  • Assay type
  • Sensitivity
    < 10 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam's Ammonia Assay Kit - Modified Berthelot - (Colorimetric) (ab102509) provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.


    Store the kit at +4°C.


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 100 tests
    Ammonia Reagent 1 Amber 1 x 8ml
    Ammonia Reagent 2 Clear 1 x 4ml
    Ammonium Chloride Standard Yellow 1 x 0.1ml
  • Research areas
  • Relevance
    Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.


  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted.

  • Ammonia measured in biological fluids showing quantity (mM) in tested samples. Samples were diluted 10-1000 fold.



ab102509 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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In response to your questions on this kit, the pH of the sample should be ˜7-8. To use mouse stool sample, add assay buffer to sample. Mix it completely either by pipetting up & down, sonicating or vortexing. Centr...

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Thank you for contacting us. I have spoken with the lab regarding the need to deproteinize mouse urine samples with this kit. They would still strongly recommned this step to attain the best possible data with this product. I hope this informatio...

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This product may be used to measure ammonium. In thisassay, the standards used are ammonium. Adjustment of the protocol is not required and detection levels will be the same.

I hope this infor...

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Thank you for your inquiry.

The most common reason for a low standard curve is a mistake when preparing the dilutions. I can tell from my on personal experience that a 10 times calculation mistake is especially easy to make.


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Thank you for calling Abcam earlier today

I have talked to the lab and the kit components are stable for at least 6 months (and up to 12 month) from the shipping date, if stored at the right temp and conditions.

Please let me know i...

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Thank you for contacting us. The kits you listed are appropriate for your samples, though we recommend ab83360 over ab102509 for assay of ammonia in urine samples. None have been published, to our knowledge. For a quote, we will need a shippin...

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Thank you for your inquiry. The lab confirmed that yes, these assay kits are compatible with serum samples from mammalian samples but they did not provide an exact protocol. I've included our general serum sample protocol below: For serum: Coll...

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