Key features and details
- Assay type: Quantitative
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture media, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 10 µM
Product nameAmmonia Assay Kit - Modified Berthelot - (Colorimetric)
See all Ammonia kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Cell culture media
Sensitivity< 10 µM
Assay time0h 30m
Ammonia Assay Kit - Modified Berthelot (Colorimetric) ab102509 provides a rapid, simple, sensitive, and reliable assay to measure ammonia.
In this non-enzymatic ammonia assay protocol, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (λ=670nm) using a plate reader.
The kit can detect less than 1 nmol(~10 µM) ammonia or ammonium using either a 96 well or 384 well microwell plate.
Ammonia assay protocol summary:
- add samples and standards to wells
- add reagent 1 and reagent 2 and incubate for 30 min
- analyze with a microplate reader
Ammonia assay kit ab83360 is an alternative to this product, and is the most popular ammonia assay kit.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 100 tests Ammonia Reagent 1 Amber 1 x 8ml Ammonia Reagent 2 Clear 1 x 4ml Ammonium Chloride Standard Yellow 1 x 0.1ml
RelevanceAmmonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.
ab102509 has been referenced in 2 publications.
- Raghavan SS et al. Development and application of a transcriptional sensor for detection of heterologous acrylic acid production in E. coli. Microb Cell Fact 18:139 (2019). PubMed: 31426802
- Wu C et al. Fluxomic Analysis Reveals Central Carbon Metabolism Adaptation for Diazotroph Azotobacter vinelandii Ammonium Excretion. Sci Rep 9:13209 (2019). PubMed: 31520074