Overview

  • Product name
    Anti-AMN antibody [EPR12015(B)]
    See all AMN primary antibodies
  • Description
    Rabbit monoclonal [EPR12015(B)] to AMN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human AMN aa 100-200 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BXU7

  • Positive control
    • Human small intestine and fetal kidney lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab170893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 47 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Function
      Necessary for efficient absorption of vitamin B12. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm.
    • Tissue specificity
      Long isoforms are highly expressed in small intestine, colon and kidney (renal proximal tubule epithelial cells). Shorter isoforms are detected at lower levels in testis, thymus and peripheral blood leukocytes.
    • Involvement in disease
      Defects in AMN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1) [MIM:261100]; also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected.
    • Sequence similarities
      Contains 1 VWFC domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5033428N14Rik antibody
      • amn antibody
      • Amnion associated transmembrane protein antibody
      • Amnionless antibody
      • Amnionless homolog antibody
      • AMNLS_HUMAN antibody
      • AV002116 antibody
      • PRO1028 antibody
      • Protein amnionless antibody
      • Type I transmembrane protein antibody
      • UNQ513/PRO1028 antibody
      • Visceral endoderm specific type 1 transmembrane protein antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from (1) Human fetal kidney lysate or (2) 1X PBS (negative control) using ab170893 at 1/1000 dilution. Detection utilised HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    • All lanes : Anti-AMN antibody [EPR12015(B)] (ab170893) at 1/1000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 47 kDa

    References

    This product has been referenced in:
    • Pannérec A  et al. Vitamin B12 deficiency and impaired expression of amnionless during aging. J Cachexia Sarcopenia Muscle 9:41-52 (2018). WB ; Rat . Read more (PubMed: 29159972) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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