RecombinantRabMAb

Recombinant Anti-AMPD1 antibody [EPR7495] (ab154814)

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Western blot - Anti-AMPD1 antibody [EPR7495] (ab154814)
  • Anti-AMPD1 antibody [EPR7495] (ab154814)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7495] to AMPD1
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-AMPD1 antibody [EPR7495]
    See all AMPD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7495] to AMPD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human AMPD1 (UniProt P23109).

  • Positive control

    • Human fetal muscle and Human skeletal muscle lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 90 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 90 kDa.
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Relevance

      AMP deaminase (AMPD) is an allosteric enzyme that plays a critical role in energy metabolism. There are three functional isoforms of AMPD. AMPD1 is the skeletal muscle-specific isoform M located in type II muscle fibers, neuromuscular junctions and in capillaries. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM). AMPDDM is a metabolic disorder resulting in exercise-related myopathy. It is characterized by exercise-induced muscle aches, cramps, and early fatigue.

    • Database links

      • Alternative names

        • Adenosine monophosphate deaminase 1 (isoform M) antibody
        • Adenosine monophosphate deaminase 1 antibody
        • AMP deaminase 1 antibody
        • AMP deaminase isoform M antibody
        • AMPD 1 antibody
        • AMPD antibody
        • Ampd01 antibody
        • MAD antibody
        • MADA antibody
        • Myoadenylate deaminase antibody
        • RATAMPD01 antibody
        • Skeletal muscle AMPD antibody
        see all

      Images

      • Western blot - Anti-AMPD1 antibody [EPR7495] (ab154814)
        Western blot - Anti-AMPD1 antibody [EPR7495] (ab154814)
        All lanes : Anti-AMPD1 antibody [EPR7495] (ab154814) at 1/1000 dilution

        Lane 1 : Human fetal muscle lysate
        Lane 2 : Human skeletal muscle lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 90 kDa

      • Anti-AMPD1 antibody [EPR7495] (ab154814)
        Anti-AMPD1 antibody [EPR7495] (ab154814)

      References (1)

      Publishing research using ab154814? Please let us know so that we can cite the reference in this datasheet.

      ab154814 has been referenced in 1 publication.

      • Plaideau C  et al. Effects of pharmacological AMP deaminase inhibition and Ampd1 deletion on nucleotide levels and AMPK activation in contracting skeletal muscle. Chem Biol 21:1497-1510 (2014). PubMed: 25459662

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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