Recombinant Anti-AMPD1 antibody [EPR7495] (ab154814)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-AMPD1 antibody [EPR7495]
See all AMPD1 primary antibodies -
Description
Rabbit monoclonal [EPR7495] to AMPD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in Human AMPD1 (UniProt P23109).
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Positive control
- Human fetal muscle and Human skeletal muscle lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7495 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab154814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 90 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Relevance
AMP deaminase (AMPD) is an allosteric enzyme that plays a critical role in energy metabolism. There are three functional isoforms of AMPD. AMPD1 is the skeletal muscle-specific isoform M located in type II muscle fibers, neuromuscular junctions and in capillaries. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM). AMPDDM is a metabolic disorder resulting in exercise-related myopathy. It is characterized by exercise-induced muscle aches, cramps, and early fatigue. -
Database links
- Entrez Gene: 270 Human
- Omim: 102770 Human
- SwissProt: P23109 Human
- Unigene: 89570 Human
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Alternative names
- Adenosine monophosphate deaminase 1 (isoform M) antibody
- Adenosine monophosphate deaminase 1 antibody
- AMP deaminase 1 antibody
see all
Images
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![Western blot - Anti-AMPD1 antibody [EPR7495] (ab154814)](https://www.abcam.com/ps/products/154/ab154814/Images/ab154814-161368-anti-ampd1-antibody-epr7495-western-blot.jpg)
Western blot - Anti-AMPD1 antibody [EPR7495] (ab154814)All lanes : Anti-AMPD1 antibody [EPR7495] (ab154814) at 1/1000 dilution
Lane 1 : Human fetal muscle lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
Datasheets and documents
References
ab154814 has not yet been referenced specifically in any publications.
