Recombinant Anti-AMPD1 antibody [EPR7495] (ab154814)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7495] to AMPD1
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-AMPD1 antibody [EPR7495]
See all AMPD1 primary antibodies -
Description
Rabbit monoclonal [EPR7495] to AMPD1 -
Host species
Rabbit -
Tested Applications & Species
Application Species WB Human -
Immunogen
Synthetic peptide corresponding to residues in Human AMPD1 (UniProt P23109).
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Positive control
- Human fetal muscle and Human skeletal muscle lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7495 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 90 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 90 kDa. |
Target
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Relevance
AMP deaminase (AMPD) is an allosteric enzyme that plays a critical role in energy metabolism. There are three functional isoforms of AMPD. AMPD1 is the skeletal muscle-specific isoform M located in type II muscle fibers, neuromuscular junctions and in capillaries. Defects in AMPD1 are the cause of adenosine monophosphate deaminase deficiency muscle type (AMPDDM). AMPDDM is a metabolic disorder resulting in exercise-related myopathy. It is characterized by exercise-induced muscle aches, cramps, and early fatigue. -
Database links
- Entrez Gene: 270 Human
- Omim: 102770 Human
- SwissProt: P23109 Human
- Unigene: 89570 Human
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Alternative names
- Adenosine monophosphate deaminase 1 (isoform M) antibody
- Adenosine monophosphate deaminase 1 antibody
- AMP deaminase 1 antibody
see all
Images
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All lanes : Anti-AMPD1 antibody [EPR7495] (ab154814) at 1/1000 dilution
Lane 1 : Human fetal muscle lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
Datasheets and documents
References (1)
ab154814 has been referenced in 1 publication.
- Plaideau C et al. Effects of pharmacological AMP deaminase inhibition and Ampd1 deletion on nucleotide levels and AMPK activation in contracting skeletal muscle. Chem Biol 21:1497-1510 (2014). PubMed: 25459662