Overview

  • Product name

  • Description

    Rabbit polyclonal to AMPD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of Human AMPD2.

  • Positive control

    • HeLa whole cell lysate (ab150035). This antibody gave a positive result when used in the following formaldehyde fixed cell line: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab135966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 101 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    AMP deaminase plays a critical role in energy metabolism.
  • Tissue specificity

    Three isoforms are present in mammals: AMP deaminase 1 is the predominant form in skeletal muscle; AMP deaminase 2 predominates in smooth muscle, non-muscle tissue, embryonic muscle and undifferentiated myoblasts; AMP deaminase 3 is found in erythrocytes.
  • Pathway

    Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.
  • Sequence similarities

    Belongs to the adenosine and AMP deaminases family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adenosine monophosphate deaminase 2 (isoform L) antibody
    • Adenosine monophosphate deaminase 2 antibody
    • adenosine monophosphate deaminase 2 isoform L antibody
    • AMP deaminase 2 antibody
    • AMP deaminase isoform L antibody
    • AMPD 2 antibody
    • AMPD antibody
    • AMPD2 antibody
    • AMPD2_HUMAN antibody
    • RP5-1160K1.5 antibody
    • SPG63 antibody
    see all

Images

  • ICC/IF image of ab135966 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135966 at 5 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-AMPD2 antibody (ab135966) at 1/500 dilution + HeLa cell lysate at 30 µg

    Predicted band size: 101 kDa

References

ab135966 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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