Anti-AMPD3 antibody [AMPD3/901] (ab233873)
Key features and details
- Mouse monoclonal [AMPD3/901] to AMPD3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-AMPD3 antibody [AMPD3/901] -
Description
Mouse monoclonal [AMPD3/901] to AMPD3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human AMPD3.
Database link: Q01432 -
Positive control
- IHC-P: Human tonsil and placenta tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
AMPD3/901 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at RT. |
Notes |
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IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 30 minutes at RT. |
Target
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Function
AMP deaminase plays a critical role in energy metabolism. -
Tissue specificity
Three isoforms are present in mammals: AMP deaminase 1 is the predominant form in skeletal muscle; AMP deaminase 2 predominates in smooth muscle, non-muscle tissue, embryonic muscle and undifferentiated myoblasts; AMP deaminase 3 is found in erythrocytes. -
Pathway
Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1. -
Involvement in disease
Defects in AMPD3 are the cause of adenosine monophosphate deaminase deficiency erythrocyte type (AMPDDE) [MIM:612874]; also known as erythrocyte AMP deaminase deficiency. AMPDDE is a metabolic disorder due to lack of activity of the erythrocyte isoform of AMP deaminase. It is a clinically asymptomatic condition characterized by a 50% increase in steady-state levels of ATP in affected cells. Individuals with complete deficiency of erythrocyte AMP deaminase are healthy and have no hematologic disorders. -
Sequence similarities
Belongs to the adenosine and AMP deaminases family. - Information by UniProt
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Database links
- Entrez Gene: 272 Human
- Omim: 102772 Human
- SwissProt: Q01432 Human
- Unigene: 501890 Human
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Alternative names
- Adenosine monophosphate deaminase (isoform E) antibody
- Adenosine monophosphate deaminase 3 antibody
- AMP aminohydrolase antibody
see all
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233873 has not yet been referenced specifically in any publications.