Key features and details
- Rabbit polyclonal to Amphiphysin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Amphiphysin antibody
See all Amphiphysin primary antibodies
DescriptionRabbit polyclonal to Amphiphysin
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human cerebral cortex and colon tissue. WB: RT4 cell lysate. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionMay participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Tissue specificityNeurons, certain endocrine cell types and spermatocytes.
Sequence similaritiesContains 1 BAR domain.
Contains 1 SH3 domain.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- Amp antibody
- AMPH 1 antibody
- AMPH antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Amphiphysin (green) using ab244375 at 4 µg/ml in ICC/IF.
Anti-Amphiphysin antibody (ab244375) at 0.4 µg/ml + RT4 (Human urinary bladder cancer cell line) cell lysate
Paraffin-embedded human cerebral cortex tissue stained for Amphiphysin using ab244375 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded human colon tissue stained for Amphiphysin using ab244375 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for Amphiphysin using ab244375 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for Amphiphysin using ab244375 at 1/1000 dilution in immunohistochemical analysis.
Low expression as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244375 has not yet been referenced specifically in any publications.