Product nameAnti-Amphiphysin antibody
See all Amphiphysin primary antibodies
DescriptionGoat polyclonal to Amphiphysin
SpecificityThis antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1) of Amphiphysin.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Dog, Human
- Mouse Brain lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab50340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 76 kDa). The observed molecular weight corresponds to earlier findings in literature (Wigge et al, Mol Biol Cell. 1997 Oct;8(10):2003-15.; PMID: 9348539). A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Tissue specificityNeurons, certain endocrine cell types and spermatocytes.
Sequence similaritiesContains 1 BAR domain.
Contains 1 SH3 domain.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- Amp antibody
- AMPH 1 antibody
- AMPH antibody
Anti-Amphiphysin antibody (ab50340) at 0.1 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 76 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
A minor band of unknown identity was also consistently observed at 45kDa. This band was successfully blocked by incubation with the immunising peptide.
ab50340 has not yet been referenced specifically in any publications.