• Product name

    Anti-Amphiphysin antibody [EP2060Y]
    See all Amphiphysin primary antibodies
  • Description

    Rabbit monoclonal [EP2060Y] to Amphiphysin
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Amphiphysin aa 250-350 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Human brain lysate, human brain tissue or SH-SYS5 cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab52646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
    • Tissue specificity

      Neurons, certain endocrine cell types and spermatocytes.
    • Sequence similarities

      Contains 1 BAR domain.
      Contains 1 SH3 domain.
    • Cellular localization

      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • Amp antibody
      • AMPH 1 antibody
      • AMPH antibody
      • AMPH_HUMAN antibody
      • AMPH1 antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen) antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) antibody
      • Amphiphysin antibody
      • Amphiphysin I antibody
      • CG8604 antibody
      • Damp antibody
      • dAmph antibody
      • Dmel\CG8604 antibody
      • HGNC:471 antibody
      • MGC49429 antibody
      • Stiff man syndrome with breast cancer 128kD autoantigen antibody
      • Stiff man syndrome with breast cancer 128kDa autoantigen antibody
      see all


    • Anti-Amphiphysin antibody [EP2060Y] (ab52646) at 1/100000 dilution + Human brain lysate at 10 µg

      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 76 kDa
      Observed band size: 128 kDa
      why is the actual band size different from the predicted?

    • Ab52646 (1/100) staining human Amphiphysin in human brain tissue by immunohistochemistry using paraffin embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


    This product has been referenced in:

    • Buser DP  et al. Quantitative proteomics reveals reduction of endocytic machinery components in gliomas. EBioMedicine 46:32-41 (2019). Read more (PubMed: 31331834) »
    • Bosch PJ  et al. Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats. PLoS One 10:e0139829 (2015). WB . Read more (PubMed: 26484527) »
    See all 3 Publications for this product

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