Product nameAnti-Amphiphysin antibody [EP2060Y]
See all Amphiphysin primary antibodies
DescriptionRabbit monoclonal [EP2060Y] to Amphiphysin
Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Amphiphysin aa 250-350 (internal sequence). The exact sequence is proprietary.
- Human brain lysate, human brain tissue or SH-SYS5 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Tissue specificityNeurons, certain endocrine cell types and spermatocytes.
Sequence similaritiesContains 1 BAR domain.
Contains 1 SH3 domain.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- Amp antibody
- AMPH 1 antibody
- AMPH antibody
Immunofluorescent staining of SH-SYS5 cells using ab52646 (1/100).
Anti-Amphiphysin antibody [EP2060Y] (ab52646) at 1/100000 dilution + Human brain lysate at 10 µg
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 76 kDa
Observed band size: 128 kDa why is the actual band size different from the predicted?
Ab52646 (1/100) staining human Amphiphysin in human brain tissue by immunohistochemistry using paraffin embedded tissue.
Overlay histogram showing SH-SY5Y cells stained with ab52646 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52646, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Bosch PJ et al. Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats. PLoS One 10:e0139829 (2015). WB . Read more (PubMed: 26484527) »
- Kumar GS et al. Quantitative proteomics for identifying biomarkers for tuberculous meningitis. Clin Proteomics 9:12 (2012). IHC . Read more (PubMed: 23198679) »