Overview

  • Product name

    Anti-Amphiphysin antibody [EP2060Y]
    See all Amphiphysin primary antibodies
  • Description

    Rabbit monoclonal [EP2060Y] to Amphiphysin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Amphiphysin aa 250-350 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Human brain lysate, human brain tissue or SH-SYS5 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
    • Tissue specificity

      Neurons, certain endocrine cell types and spermatocytes.
    • Sequence similarities

      Contains 1 BAR domain.
      Contains 1 SH3 domain.
    • Cellular localization

      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • Amp antibody
      • AMPH 1 antibody
      • AMPH antibody
      • AMPH_HUMAN antibody
      • AMPH1 antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen) antibody
      • Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) antibody
      • Amphiphysin antibody
      • Amphiphysin I antibody
      • CG8604 antibody
      • Damp antibody
      • dAmph antibody
      • Dmel\CG8604 antibody
      • HGNC:471 antibody
      • MGC49429 antibody
      • Stiff man syndrome with breast cancer 128kD autoantigen antibody
      • Stiff man syndrome with breast cancer 128kDa autoantigen antibody
      see all

    Images

    • Anti-Amphiphysin antibody [EP2060Y] (ab52646) at 1/100000 dilution + Human brain lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 76 kDa
      Observed band size: 128 kDa
      why is the actual band size different from the predicted?

    • Ab52646 (1/100) staining human Amphiphysin in human brain tissue by immunohistochemistry using paraffin embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Buser DP  et al. Quantitative proteomics reveals reduction of endocytic machinery components in gliomas. EBioMedicine 46:32-41 (2019). Read more (PubMed: 31331834) »
    • Bosch PJ  et al. Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats. PLoS One 10:e0139829 (2015). WB . Read more (PubMed: 26484527) »
    See all 3 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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