Key features and details
- Rabbit monoclonal [EP2061Y] to Amphiphysin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Amphiphysin antibody [EP2061Y]
See all Amphiphysin primary antibodies
DescriptionRabbit monoclonal [EP2061Y] to Amphiphysin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Amphiphysin (C terminal). The exact sequence is proprietary.
- Human, mouse and rat brain tissue lysates; human brain tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20000 - 1/100000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Tissue specificityNeurons, certain endocrine cell types and spermatocytes.
Sequence similaritiesContains 1 BAR domain.
Contains 1 SH3 domain.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- Amp antibody
- AMPH 1 antibody
- AMPH antibody
ab76124 at 1/100 dilution, staining Amphiphysin in human brain by Immunohistochemistry using paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
All lanes : Anti-Amphiphysin antibody [EP2061Y] (ab76124) at 1/100000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 76 kDa
Observed band size: 128 kDa why is the actual band size different from the predicted?
ab76124 has not yet been referenced specifically in any publications.