Anti-Amphiregulin antibody (ab180722)
Key features and details
- Rabbit polyclonal to Amphiregulin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Amphiregulin antibody
See all Amphiregulin primary antibodies -
Description
Rabbit polyclonal to Amphiregulin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human Amphiregulin aa 20-252. Mature Protein without signal peptide.
Database link: P15514 -
Positive control
- WB: MCF7, A549, HT29 and Mouse brain lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 28 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 28 kDa. |
Target
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Function
Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells. -
Sequence similarities
Belongs to the amphiregulin family.
Contains 1 EGF-like domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 374 Human
- Entrez Gene: 727738 Human
- Entrez Gene: 11839 Mouse
- Omim: 104640 Human
- SwissProt: P15514 Human
- SwissProt: P31955 Mouse
- Unigene: 270833 Human
- Unigene: 645475 Human
see all -
Alternative names
- A REG antibody
- Amphiregulin antibody
- Amphiregulin B antibody
see all
Images
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Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution + A549 (Human lung carcinoma cell line) whole cell lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer: 3% nonfat dry milk in TBST.
PMID: 9642297.
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Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution + Mouse brain lysate at 25 µg
Secondary
HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 300 secondsBlocking buffer: 3% nonfat dry milk in TBST.
PMID: 9642297.
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All lanes : Anti-Amphiregulin antibody (ab180722) at 1/500 dilution
Lane 1 : MCF7 cell extracts
Lane 2 : A549 cell extracts
Lane 3 : HT29 cell extracts
Predicted band size: 28 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab180722 has been referenced in 3 publications.
- Geng H et al. HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway. Mol Med Rep 24:N/A (2021). PubMed: 34396425
- Chen S et al. Renal subcapsular delivery of PGE2 promotes kidney repair by activating endogenous Sox9+ stem cells. iScience 24:103243 (2021). PubMed: 34746706
- Dong X et al. Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis. Front Oncol 10:160 (2020). PubMed: 32154166