Overview

  • Product name
    Anti-Amphiregulin antibody
    See all Amphiregulin primary antibodies
  • Description
    Rabbit polyclonal to Amphiregulin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Amphiregulin.

  • Positive control
    • LS174T cells
  • General notes


    Amphiregulin binds to EGF Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84 amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252 amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGF alpha, AR, HB EGF, cripto 1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Constituents: 0.0268% PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Amphiregulin binds to EGF Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84 amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252 amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGF alpha, AR, HB EGF, cripto 1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 10 µg/mg of lysate.

With protein A, native.

Target

  • Function
    Bifunctional growth-modulating glycoprotein. Inhibits growth of several human carcinoma cells in culture and stimulates proliferation of human fibroblasts and certain other tumor cells.
  • Sequence similarities
    Belongs to the amphiregulin family.
    Contains 1 EGF-like domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A REG antibody
    • Amphiregulin antibody
    • Amphiregulin B antibody
    • AR antibody
    • AREG antibody
    • AREG_HUMAN antibody
    • AREGB antibody
    • Colorectum cell derived growth factor antibody
    • Colorectum cell-derived growth factor antibody
    • CRDGF antibody
    • MGC13647 antibody
    • Schwannoma derived growth factor antibody
    • SDGF antibody
    see all

References

ab2993 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. We would like to confirm that this primary antibody is un-conjugated therefore you need an HRP-linked secondary antibody. The recommended compatible secondary antibodies are listed on the bottom section of the on-line datasheet. This antibody was made against N- terminus peptide (19-mer peptide) aa sequence VKPPONKTESENTSDKKPKR. As the datasheet suggests, in immunoprecipitation this antibody reacts with native protein. We have also tested this antibody in WB with native protein MW 18.5 kDa and 22.5 kDa. We would suggest perhaps checking the sequence of pro vs active protein using the database of Swiss-Prot.

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Answer

Thank you for your enquiry. The ab2993 Amphiregulin antibody recognizes a synthetic 19 mer peptide, corresponding to amino acids 8 - 26 (VKPPQNKTESENTSDKPKR) from the secretedamphiregulin of human origin. If that amino acid sequence is present in the pro and the active forms of amphiregulin, the Ab detects both. We don't offer any other Ab for amphiregulin beside the Ab2993. However, when you do a search on the Abcam's website (write amphiregulin in the search window on the upper left side), you will see this line " Abcam catalog search results | The world's antibody gateway (external search results)" soon after the yellow and before the light blue lanes. Click on "World's antibody gateway" and you will get a list of other amphiregulin Ab from other suppliers. I hope this information is helpful, and more importantly, good luck with your research!

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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